Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 213962..214604 Replicon chromosome
Accession NZ_CP011155
Organism Bacillus cereus strain HN001

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag WR52_RS01195 Protein ID WP_000635963.1
Coordinates 214254..214604 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag WR52_RS01190 Protein ID WP_000004570.1
Coordinates 213962..214249 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WR52_RS01165 209279..210241 + 963 WP_063535496.1 UV DNA damage repair endonuclease UvsE -
WR52_RS01170 210234..210806 - 573 WP_000908524.1 rhomboid family intramembrane serine protease -
WR52_RS01175 210900..211259 + 360 WP_063535497.1 holo-ACP synthase -
WR52_RS01180 211416..212366 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
WR52_RS01185 212484..213653 + 1170 WP_000390615.1 alanine racemase -
WR52_RS01190 213962..214249 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
WR52_RS01195 214254..214604 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
WR52_RS01200 214672..216840 + 2169 WP_000426242.1 RNA-binding transcriptional accessory protein -
WR52_RS29950 216898..217014 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
WR52_RS01205 217210..217668 + 459 WP_063535498.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T53583 WP_000635963.1 NZ_CP011155:214254-214604 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T53583 NZ_CP011155:214254-214604 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT53583 WP_000004570.1 NZ_CP011155:213962-214249 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT53583 NZ_CP011155:213962-214249 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References