Detailed information of TA system TA053582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2428220..2429198
• Replicon: chromosome
• Accession: NZ_CP011153
• Organism: Bacillus cereus strain CMCC P0011

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: WR51_RS12190
• Protein ID: WP_000624977.1
• Coordinates: 2428220..2428957 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: WR51_RS12195
• Protein ID: WP_000237819.1
• Coordinates: 2429070..2429198 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WR51_RS12170	2423862..2424644	+	783	WP_063547282.1	class I SAM-dependent methyltransferase	-
WR51_RS12175	2424802..2426487	-	1686	WP_063547283.1	alpha-keto acid decarboxylase family protein	-
WR51_RS12180	2426595..2427077	+	483	WP_000191889.1	MarR family winged helix-turn-helix transcriptional regulator	-
WR51_RS12185	2427244..2427981	+	738	WP_063547284.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
WR51_RS12190	2428220..2428957	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
WR51_RS12195	2429070..2429198	+	129	WP_000237819.1	hypothetical protein	Antitoxin
WR51_RS32540	2429271..2429447	+	177	WP_063547285.1	hypothetical protein	-
WR51_RS12205	2429491..2429766	-	276	WP_063547286.1	hypothetical protein	-
WR51_RS32545	2429793..2430587	-	795	WP_176328690.1	hypothetical protein	-
WR51_RS12215	2430648..2431628	-	981	WP_063547287.1	WXG100 family type VII secretion target	-
WR51_RS12220	2431959..2432135	+	177	WP_000852629.1	stage II sporulation protein SB	-
WR51_RS12225	2432152..2432541	-	390	WP_000713869.1	YxeA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T53582 WP_000624977.1 NZ_CP011153:2428220-2428957 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T53582 NZ_CP011153:2428220-2428957 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCTGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT53582 WP_000237819.1 NZ_CP011153:2429070-2429198 [Bacillus cereus]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT53582 NZ_CP011153:2429070-2429198 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGGTAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2