53581

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 239378..240020 Replicon chromosome
Accession NZ_CP011153
Organism Bacillus cereus strain CMCC P0011

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag WR51_RS01335 Protein ID WP_000635965.1
Coordinates 239670..240020 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag WR51_RS01330 Protein ID WP_000004570.1
Coordinates 239378..239665 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WR51_RS01305 234695..235657 + 963 WP_000961144.1 UV DNA damage repair endonuclease UvsE -
WR51_RS01310 235650..236222 - 573 WP_000908526.1 rhomboid family intramembrane serine protease -
WR51_RS01315 236316..236675 + 360 WP_000583419.1 holo-ACP synthase -
WR51_RS01320 236832..237782 + 951 WP_002094231.1 outer membrane lipoprotein carrier protein LolA -
WR51_RS01325 237900..239069 + 1170 WP_000390593.1 alanine racemase -
WR51_RS01330 239378..239665 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
WR51_RS01335 239670..240020 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
WR51_RS01340 240089..242257 + 2169 WP_000426207.1 RNA-binding transcriptional accessory protein -
WR51_RS30005 242315..242431 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
WR51_RS01345 242627..243085 + 459 WP_000503730.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T53581 WP_000635965.1 NZ_CP011153:239670-240020 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T53581 NZ_CP011153:239670-240020 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT53581 WP_000004570.1 NZ_CP011153:239378-239665 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT53581 NZ_CP011153:239378-239665 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References