Detailed information of TA system TA053580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2442958..2443936
• Replicon: chromosome
• Accession: NZ_CP011151
• Organism: Bacillus cereus strain CMCC P0021

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: WR47_RS12190
• Protein ID: WP_000624977.1
• Coordinates: 2442958..2443695 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: WR47_RS12195
• Protein ID: WP_000237819.1
• Coordinates: 2443808..2443936 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WR47_RS12170	2438600..2439382	+	783	WP_063547282.1	class I SAM-dependent methyltransferase	-
WR47_RS12175	2439540..2441225	-	1686	WP_063547283.1	alpha-keto acid decarboxylase family protein	-
WR47_RS12180	2441333..2441815	+	483	WP_000191889.1	MarR family winged helix-turn-helix transcriptional regulator	-
WR47_RS12185	2441982..2442719	+	738	WP_063547284.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
WR47_RS12190	2442958..2443695	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
WR47_RS12195	2443808..2443936	+	129	WP_000237819.1	hypothetical protein	Antitoxin
WR47_RS32555	2444009..2444185	+	177	WP_063547285.1	hypothetical protein	-
WR47_RS12205	2444229..2444504	-	276	WP_063547286.1	hypothetical protein	-
WR47_RS32560	2444531..2445325	-	795	WP_176328690.1	hypothetical protein	-
WR47_RS12215	2445386..2446366	-	981	WP_063547287.1	WXG100 family type VII secretion target	-
WR47_RS12220	2446697..2446873	+	177	WP_000852629.1	stage II sporulation protein SB	-
WR47_RS12225	2446890..2447279	-	390	WP_000713869.1	YxeA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T53580 WP_000624977.1 NZ_CP011151:2442958-2443695 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T53580 NZ_CP011151:2442958-2443695 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCTGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT53580 WP_000237819.1 NZ_CP011151:2443808-2443936 [Bacillus cereus]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT53580 NZ_CP011151:2443808-2443936 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGGTAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2