Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 240802..241444 Replicon chromosome
Accession NZ_CP011145
Organism Bacillus cereus strain FORC_013

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag FORC13_RS01400 Protein ID WP_000635963.1
Coordinates 241094..241444 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FORC13_RS01395 Protein ID WP_000004570.1
Coordinates 240802..241089 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC13_RS01370 236119..237081 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
FORC13_RS01375 237074..237646 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
FORC13_RS01380 237740..238099 + 360 WP_059303372.1 holo-ACP synthase -
FORC13_RS01385 238256..239206 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
FORC13_RS01390 239324..240493 + 1170 WP_000390615.1 alanine racemase -
FORC13_RS01395 240802..241089 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FORC13_RS01400 241094..241444 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FORC13_RS01405 241512..243680 + 2169 WP_000426242.1 RNA-binding transcriptional accessory protein -
FORC13_RS01410 243738..243854 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FORC13_RS01415 244050..244508 + 459 WP_000344231.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T53559 WP_000635963.1 NZ_CP011145:241094-241444 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T53559 NZ_CP011145:241094-241444 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT53559 WP_000004570.1 NZ_CP011145:240802-241089 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT53559 NZ_CP011145:240802-241089 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References