Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 40259..40866 Replicon plasmid pVR50B
Accession NZ_CP011136
Organism Escherichia coli VR50

Toxin (Protein)


Gene name doc Uniprot ID B7LIH8
Locus tag ECVR50_RS25725 Protein ID WP_000673985.1
Coordinates 40259..40645 (-) Length 129 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID B7LIH9
Locus tag ECVR50_RS25730 Protein ID WP_000493532.1
Coordinates 40642..40866 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECVR50_RS25705 35370..35558 + 189 WP_000744815.1 hypothetical protein -
ECVR50_RS25710 35686..36801 + 1116 WP_001673230.1 hypothetical protein -
ECVR50_RS25715 37074..37757 + 684 WP_000200414.1 lasso peptide biosynthesis B2 protein -
ECVR50_RS25720 37757..39481 + 1725 WP_001554923.1 ABC transporter ATP-binding protein/permease -
ECVR50_RS25725 40259..40645 - 387 WP_000673985.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
ECVR50_RS25730 40642..40866 - 225 WP_000493532.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ECVR50_RS25735 41151..41948 - 798 WP_001554960.1 IS21-like element IS21 family helper ATPase IstB -
ECVR50_RS25745 41948..43120 - 1173 Protein_42 IS21-like element IS21 family transposase -
ECVR50_RS30035 43213..43467 + 255 Protein_43 hypothetical protein -
ECVR50_RS25755 43533..43742 - 210 WP_024192676.1 hypothetical protein -
ECVR50_RS25760 44285..44632 - 348 WP_023144239.1 metalloregulator ArsR/SmtB family transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - senB 1..97864 97864


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(11-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-52)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14370.62 Da        Isoelectric Point: 7.0732

>T53553 WP_000673985.1 NZ_CP011136:c40645-40259 [Escherichia coli VR50]
MKFVSSQEVIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYHYEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVA
QVFLKRNGVHIISSRISFDEMQIIALNAATGEYNWKMVSDHLKAIILN

Download         Length: 387 bp

>T53553 NZ_CP011136:c40645-40259 [Escherichia coli VR50]
ATGAAATTCGTTAGTTCACAGGAAGTGATTGAGTTTCATGACCGCTTGATTTCGCGTGATGGTGGCGTGGCGGGTATGCC
AGAACCCGGTCGCGCTGACGCGATCATTCATCGTGTTTTGAACATGTATCATTATGAAGGTGTGACAGATATTATGGATC
TGGCTGCCGTGTATTTAGTTGCTATTGCTCGTGGTCACATTTTCAACGATGCAAATAAGCGAACAGCCTTGTTTGTTGCA
CAGGTTTTTTTAAAACGGAATGGTGTACATATTATTAGTTCACGTATTTCGTTTGATGAAATGCAAATTATCGCTTTGAA
TGCTGCAACAGGAGAATATAACTGGAAGATGGTTTCTGACCATCTTAAAGCCATTATTCTAAATTAG

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8361.39 Da        Isoelectric Point: 5.6713

>AT53553 WP_000493532.1 NZ_CP011136:c40866-40642 [Escherichia coli VR50]
MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDAFERYRKAALDAEFAEIMQSHGTAYTELSKR

Download         Length: 225 bp

>AT53553 NZ_CP011136:c40866-40642 [Escherichia coli VR50]
ATGTTTACATGTAACTATAGCCATGCTCGCGATAACCTGGCCTCGTTGCTCGAGCTGGCGGTTGATGAAAATCAGCCTGT
TGAGATTCAGCGCCGTAAAGGTGGCGCGGCTGTACTAGTTGATAAAGACGCTTTTGAACGATACCGTAAAGCGGCTCTTG
ATGCTGAGTTTGCTGAGATCATGCAAAGTCATGGGACGGCCTATACGGAGCTTTCTAAGCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A086V9Y9

Antitoxin

Source ID Structure
AlphaFold DB A0A086V9Y8

References