Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 31918..32443 Replicon plasmid pVR50B
Accession NZ_CP011136
Organism Escherichia coli VR50

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag ECVR50_RS25670 Protein ID WP_001159871.1
Coordinates 31918..32223 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag ECVR50_RS25675 Protein ID WP_000813630.1
Coordinates 32225..32443 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECVR50_RS25655 27874..29079 - 1206 WP_001442122.1 AAA family ATPase -
ECVR50_RS25660 29700..30431 + 732 WP_000504263.1 replication initiation protein -
ECVR50_RS25665 31111..31917 - 807 WP_000016968.1 site-specific integrase -
ECVR50_RS25670 31918..32223 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
ECVR50_RS25675 32225..32443 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ECVR50_RS25685 33038..33307 + 270 WP_001554929.1 hypothetical protein -
ECVR50_RS25690 33295..33891 + 597 WP_001554928.1 hypothetical protein -
ECVR50_RS25695 33909..34256 - 348 WP_001554927.1 hypothetical protein -
ECVR50_RS25700 34367..34714 + 348 WP_001554926.1 hypothetical protein -
ECVR50_RS28385 34732..35158 - 427 Protein_34 hypothetical protein -
ECVR50_RS25705 35370..35558 + 189 WP_000744815.1 hypothetical protein -
ECVR50_RS25710 35686..36801 + 1116 WP_001673230.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - senB 1..97864 97864


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T53552 WP_001159871.1 NZ_CP011136:c32223-31918 [Escherichia coli VR50]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T53552 NZ_CP011136:c32223-31918 [Escherichia coli VR50]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT53552 WP_000813630.1 NZ_CP011136:c32443-32225 [Escherichia coli VR50]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT53552 NZ_CP011136:c32443-32225 [Escherichia coli VR50]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References