Detailed information of TA system TA053551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 58359..58789
• Replicon: plasmid pVR50A
• Accession: NZ_CP011135
• Organism: Escherichia coli VR50

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: ECVR50_RS29645
• Protein ID: WP_001388374.1
• Coordinates: 58359..58523 (-)
• Length: 55 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A6Y0EDY2
• Locus tag: ECVR50_RS25435
• Protein ID: WP_000079939.1
• Coordinates: 58520..58789 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECVR50_RS25405	53527..54231	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
ECVR50_RS25410	54304..55107	+	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
ECVR50_RS25415	55107..55943	+	837	WP_000480972.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
ECVR50_RS25420	56050..56454	-	405	Protein_78	IS91 family transposase	-
ECVR50_RS25425	56566..56946	-	381	WP_001696636.1	HTH domain-containing protein	-
ECVR50_RS25430	57388..58311	-	924	WP_001415483.1	hypothetical protein	-
ECVR50_RS29645	58359..58523	-	165	WP_001388374.1	hypothetical protein	Toxin
ECVR50_RS25435	58520..58789	-	270	WP_000079939.1	hypothetical protein	Antitoxin
ECVR50_RS25440	59705..60562	-	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
ECVR50_RS25445	60555..60629	-	75	WP_001365705.1	RepA leader peptide Tap	-
ECVR50_RS25450	60867..61121	-	255	WP_000083830.1	replication regulatory protein RepA	-
ECVR50_RS25455	61361..61951	-	591	WP_000766807.1	DUF2726 domain-containing protein	-
ECVR50_RS25460	61989..62198	-	210	WP_001298565.1	hemolysin expression modulator Hha	-
ECVR50_RS25465	62244..62705	-	462	WP_001233838.1	thermonuclease family protein	-
ECVR50_RS25470	62950..63162	-	213	WP_001309245.1	ANR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / blaTEM-1B / aph(3'')-Ib / aph(6)-Id	-	1..70533	70533

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 55 a.a.
• Molecular weight: 6604.73 Da
• Isoelectric Point: 7.1989

>T53551 WP_001388374.1 NZ_CP011135:c58523-58359 [Escherichia coli VR50]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYFGRTHRLTPVLHLYYM

Nucleotide

• Length: 165 bp

>T53551 NZ_CP011135:c58523-58359 [Escherichia coli VR50]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAATACATTGCGGAGCAGAACCTGAT
AGCAGCCATTGAACTGGACGAACGGATTGGTTATTTCGGACGAACGCACCGGCTTACGCCGGTTTTACATCTGTACTACA
TGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10233.54 Da
• Isoelectric Point: 6.7263

>AT53551 WP_000079939.1 NZ_CP011135:c58789-58520 [Escherichia coli VR50]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT53551 NZ_CP011135:c58789-58520 [Escherichia coli VR50]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCTGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGTCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCACGGCGATGTACTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGAGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6Y0EDY2