Detailed information of TA system TA053550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4914237..4915051
• Replicon: chromosome
• Accession: NZ_CP011134
• Organism: Escherichia coli VR50

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: ECVR50_RS24530
• Protein ID: WP_001054376.1
• Coordinates: 4914237..4914494 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: ECVR50_RS24535
• Protein ID: WP_001309181.1
• Coordinates: 4914506..4915051 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECVR50_RS24505	4909525..4910631	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
ECVR50_RS24510	4910696..4911676	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
ECVR50_RS24515	4912259..4913499	-	1241	Protein_4594	helicase YjhR	-
ECVR50_RS29575	4913615..4913860	+	246	Protein_4595	GNAT family N-acetyltransferase	-
ECVR50_RS24530	4914237..4914494	+	258	WP_001054376.1	hypothetical protein	Toxin
ECVR50_RS24535	4914506..4915051	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
ECVR50_RS24540	4915107..4915853	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
ECVR50_RS29065	4916022..4916240	+	219	Protein_4599	hypothetical protein	-
ECVR50_RS30375	4916278..4916394	+	117	Protein_4600	VOC family protein	-
ECVR50_RS24545	4916639..4917760	+	1122	WP_000010829.1	M42 family metallopeptidase	-
ECVR50_RS24550	4917757..4918035	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
ECVR50_RS24555	4918047..4919360	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4904022..4941102	37080

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T53550 WP_001054376.1 NZ_CP011134:4914237-4914494 [Escherichia coli VR50]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T53550 NZ_CP011134:4914237-4914494 [Escherichia coli VR50]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT53550 WP_001309181.1 NZ_CP011134:4914506-4915051 [Escherichia coli VR50]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT53550 NZ_CP011134:4914506-4915051 [Escherichia coli VR50]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8