Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3211383..3212037 Replicon chromosome
Accession NZ_CP011134
Organism Escherichia coli VR50

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ECVR50_RS16010 Protein ID WP_000244777.1
Coordinates 3211383..3211790 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECVR50_RS16015 Protein ID WP_000354046.1
Coordinates 3211771..3212037 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECVR50_RS15990 3207340..3209073 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
ECVR50_RS15995 3209079..3209789 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECVR50_RS16000 3209814..3210710 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECVR50_RS16005 3210822..3211343 + 522 WP_001055874.1 flavodoxin FldB -
ECVR50_RS16010 3211383..3211790 - 408 WP_000244777.1 toxin CptA Toxin
ECVR50_RS16015 3211771..3212037 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECVR50_RS16020 3212280..3213260 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
ECVR50_RS16025 3213456..3214115 - 660 WP_000250274.1 hemolysin III family protein -
ECVR50_RS16030 3214279..3214590 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
ECVR50_RS16035 3214635..3216068 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
ECVR50_RS16040 3216125..3216868 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T53544 WP_000244777.1 NZ_CP011134:c3211790-3211383 [Escherichia coli VR50]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T53544 NZ_CP011134:c3211790-3211383 [Escherichia coli VR50]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT53544 WP_000354046.1 NZ_CP011134:c3212037-3211771 [Escherichia coli VR50]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT53544 NZ_CP011134:c3212037-3211771 [Escherichia coli VR50]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References