53528

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 15331..15881 Replicon plasmid unnamed
Accession NZ_CP011133
Organism Citrobacter amalonaticus Y19

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0F6TZV8
Locus tag F384_RS26230 Protein ID WP_046498752.1
Coordinates 15331..15639 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0F6RIT5
Locus tag F384_RS26235 Protein ID WP_046498757.1
Coordinates 15642..15881 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F384_RS26185 10390..10728 + 339 WP_046498719.1 hypothetical protein -
F384_RS26190 10953..11264 + 312 WP_046498724.1 hypothetical protein -
F384_RS26195 11304..11612 + 309 WP_046498729.1 hypothetical protein -
F384_RS26200 11976..12302 - 327 WP_046498732.1 hypothetical protein -
F384_RS26205 12387..12785 - 399 WP_046498736.1 hypothetical protein -
F384_RS26210 12796..13038 - 243 WP_046499465.1 hypothetical protein -
F384_RS26215 13248..13670 - 423 Protein_21 hypothetical protein -
F384_RS26220 13753..14370 - 618 WP_046498741.1 hypothetical protein -
F384_RS26225 14443..14997 - 555 WP_046498747.1 hypothetical protein -
F384_RS30200 15152..15307 - 156 WP_167337386.1 hypothetical protein -
F384_RS26230 15331..15639 - 309 WP_046498752.1 CcdB family protein Toxin
F384_RS26235 15642..15881 - 240 WP_046498757.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
F384_RS30245 16021..16485 - 465 WP_193388325.1 hypothetical protein -
F384_RS26245 16482..17153 - 672 WP_046498763.1 hypothetical protein -
F384_RS26250 17614..18255 - 642 WP_046498769.1 hypothetical protein -
F384_RS26255 18559..19017 - 459 WP_046498772.1 hypothetical protein -
F384_RS26260 19290..19985 - 696 WP_046498775.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..290993 290993


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(9-79)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11282.11 Da        Isoelectric Point: 7.3147

>T53528 WP_046498752.1 NZ_CP011133:c15639-15331 [Citrobacter amalonaticus Y19]
MQYTVYRNTGNSQAYPYLLDIQSDIIGELNTRLVIPLHLLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKGAVDFLLDGF

Download         Length: 309 bp

>T53528 NZ_CP011133:c15639-15331 [Citrobacter amalonaticus Y19]
ATGCAATACACCGTTTACCGCAATACCGGCAATAGCCAGGCATATCCTTACCTGCTGGATATACAGAGCGACATCATTGG
TGAGTTGAATACCCGTCTGGTGATCCCGCTTCATCTGCTGAAAAAAGGAGCCTCGGCCCCGGTTGCGCGGTTAACTCCGG
TTATTCAGGTGGAAGGGAATGACGTTATCCTGATGACACATGAAATGGCCTCGGTTCGCGTGAAGCAGCTTGGTCAGGCG
GTCATGGATGCATCTCCGTTCCGGCACACTATCAAAGGCGCTGTGGACTTCCTGCTGGATGGTTTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8843.01 Da        Isoelectric Point: 8.6598

>AT53528 WP_046498757.1 NZ_CP011133:c15881-15642 [Citrobacter amalonaticus Y19]
MQTTAARQKKTVSVTLEPMLVQQARDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Download         Length: 240 bp

>AT53528 NZ_CP011133:c15881-15642 [Citrobacter amalonaticus Y19]
ATGCAGACTACAGCTGCTCGCCAGAAAAAAACCGTCAGTGTCACTCTTGAGCCAATGCTTGTACAGCAGGCAAGAGACGC
TGGGATTAACTTGTCCGCCACGCTGGCAGAAGCGCTTCAGAGCAAACTGAAGGCCAGTGCTGCGGAAGAATGGAAACGAC
AGAATCGTGCTGGTATTCAGGAGTTGAACCGCATCACTGAAGAGCATGGGTTACTGTCTGACGAATACAGGACGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6TZV8

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6RIT5

References