53526

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 5322548..5323227 Replicon chromosome
Accession NZ_CP011132
Organism Citrobacter amalonaticus Y19

Toxin (Protein)


Gene name ykfI Uniprot ID A0A7D6Z078
Locus tag F384_RS29545 Protein ID WP_048215514.1
Coordinates 5322886..5323227 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0F6RHY7
Locus tag F384_RS24815 Protein ID WP_046495841.1
Coordinates 5322548..5322865 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F384_RS24780 5318107..5318940 + 834 WP_046495809.1 DUF945 domain-containing protein -
F384_RS24785 5319142..5319846 + 705 WP_046495813.1 WYL domain-containing protein -
F384_RS24790 5319843..5320457 + 615 WP_046495819.1 hypothetical protein -
F384_RS24795 5320546..5320956 + 411 WP_046495825.1 hypothetical protein -
F384_RS24800 5321034..5321270 + 237 WP_046495830.1 DUF905 domain-containing protein -
F384_RS24805 5321349..5321807 + 459 WP_046495834.1 antirestriction protein -
F384_RS24810 5321823..5322299 + 477 WP_046495838.1 RadC family protein -
F384_RS29540 5322308..5322529 + 222 WP_000691995.1 DUF987 domain-containing protein -
F384_RS24815 5322548..5322865 + 318 WP_046495841.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
F384_RS29545 5322886..5323227 + 342 WP_048215514.1 TA system toxin CbtA family protein Toxin
F384_RS24825 5323343..5324176 + 834 WP_046495848.1 DUF4942 domain-containing protein -
F384_RS24830 5324498..5325934 + 1437 WP_046495851.1 integrase family protein -
F384_RS24835 5325924..5326607 + 684 WP_046495854.1 hypothetical protein -
F384_RS24840 5326748..5326954 + 207 WP_046495857.1 helix-turn-helix domain-containing protein -
F384_RS29550 5327436..5327627 - 192 WP_080950105.1 toxin-antitoxin system HicB family antitoxin -
F384_RS24845 5327761..5328012 + 252 WP_046495862.1 host cell division inhibitor Icd-like protein -
F384_RS24850 5328005..5328196 + 192 WP_046495865.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - rfaE 5310919..5385844 74925


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(2-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13014.97 Da        Isoelectric Point: 10.4182

>T53526 WP_048215514.1 NZ_CP011132:5322886-5323227 [Citrobacter amalonaticus Y19]
MKPQSAATSRAVKPCLSPVAIWQILLTRLLEQHYGLTLNDTPFRDESVIQEHIDAGITLANAVNFLVEKYELVRIDRRRF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T53526 NZ_CP011132:5322886-5323227 [Citrobacter amalonaticus Y19]
ATGAAACCTCAATCTGCAGCAACTTCGCGGGCGGTAAAACCCTGCCTGTCACCCGTGGCTATCTGGCAAATTCTACTGAC
ACGCCTGCTGGAACAACACTACGGCCTGACCCTGAACGACACGCCGTTCAGGGATGAATCCGTTATACAGGAACATATCG
ATGCAGGTATCACGCTGGCCAATGCCGTTAACTTTCTGGTGGAAAAATACGAGCTTGTTCGTATCGATCGCAGAAGGTTT
AGTTGGCAGGAACAATCACCTTATCTTCGGGCAGCAGATATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11869.49 Da        Isoelectric Point: 6.6245

>AT53526 WP_046495841.1 NZ_CP011132:5322548-5322865 [Citrobacter amalonaticus Y19]
MSNITWGLQRDITPRLGTRLVQEGNRLHYLADRASITGKFSDAECQKLDETFPHFISQMESMLTSGELNPLHVHCVTLYH
TGFTCEADTLGSCGYVYIAIYPTKR

Download         Length: 318 bp

>AT53526 NZ_CP011132:5322548-5322865 [Citrobacter amalonaticus Y19]
ATGAGCAACATCACTTGGGGCTTGCAGCGCGATATCACGCCGCGCCTGGGAACACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTACCTAGCTGACCGGGCCAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCAGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGACCTCCGGTGAACTGAATCCCCTACACGTTCATTGCGTCACCCTGTACCAC
ACCGGTTTTACCTGCGAAGCCGACACCCTCGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTAAGCGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6Z078

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6RHY7

References