Detailed information of TA system TA053522

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2837908..2838693
• Replicon: chromosome
• Accession: NZ_CP011132
• Organism: Citrobacter amalonaticus Y19

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0F6TVT5
• Locus tag: F384_RS13105
• Protein ID: WP_046483405.1
• Coordinates: 2838316..2838693 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0F6RFT5
• Locus tag: F384_RS13100
• Protein ID: WP_046483403.1
• Coordinates: 2837908..2838267 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F384_RS13070	2833405..2834700	+	1296	WP_046483389.1	hypothetical protein	-
F384_RS13075	2834797..2835330	+	534	WP_046483391.1	hypothetical protein	-
F384_RS13080	2835502..2836320	+	819	WP_005120561.1	DUF945 domain-containing protein	-
F384_RS13085	2836673..2837158	+	486	WP_046483396.1	antirestriction protein	-
F384_RS13090	2837169..2837648	+	480	WP_046483399.1	DNA repair protein RadC	-
F384_RS13095	2837662..2837883	+	222	WP_046483401.1	DUF987 domain-containing protein	-
F384_RS13100	2837908..2838267	+	360	WP_046483403.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F384_RS13105	2838316..2838693	+	378	WP_046483405.1	TA system toxin CbtA family protein	Toxin
F384_RS13110	2838690..2839181	+	492	WP_046483407.1	hypothetical protein	-
F384_RS13115	2839211..2839414	+	204	WP_046483411.1	DUF957 domain-containing protein	-
F384_RS13120	2839495..2840343	+	849	WP_046483413.1	DUF4942 domain-containing protein	-
F384_RS13125	2840929..2841807	+	879	WP_046483417.1	nucleoside deaminase	-
F384_RS13130	2841884..2842354	+	471	WP_046483419.1	GNAT family N-acetyltransferase	-
F384_RS13135	2842412..2843011	-	600	WP_046483421.1	DUF1062 domain-containing protein	-
F384_RS13140	2843304..2843555	+	252	WP_046483423.1	Zn-finger protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14077.03 Da
• Isoelectric Point: 8.0487

>T53522 WP_046483405.1 NZ_CP011132:2838316-2838693 [Citrobacter amalonaticus Y19]
MQTQPVPPTREVSPRPSPVAIWQRLLSHLLDRHYGLTLSDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRQGF
SAETQSPLLRSIDILRARKATGLMTRNSYKVVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T53522 NZ_CP011132:2838316-2838693 [Citrobacter amalonaticus Y19]
ATGCAAACACAACCTGTTCCCCCGACACGGGAGGTTTCACCACGCCCGTCACCCGTGGCGATATGGCAGCGCCTTCTGAG
CCACCTGCTGGATCGTCACTACGGCCTTACGCTCAGCGATACGCCGTTTGGCAATGATGGCGTGATTCAGGAGCATATCG
ACGCTGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAAAAATACGATTTGGTTCGCACAGATCGTCAGGGCTTC
AGTGCAGAAACACAGTCGCCGCTTCTTAGAAGTATCGATATTCTCCGCGCCCGGAAAGCGACAGGGTTAATGACCCGCAA
CAGCTACAAAGTGGTGACCGACATCACCACCGGCAAATACCGTGAGGTGCAGCCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13291.23 Da
• Isoelectric Point: 6.4622

>AT53522 WP_046483403.1 NZ_CP011132:2837908-2838267 [Citrobacter amalonaticus Y19]
MSKKTTITTNDVSEPWWGLRRSISPCFGARLVQEANCLHYLADRTSITGQFSDADLCHLDQAFPLLLKQLELMLTSGELT
PRYQHRVTLHAKGLTCEADTLGSCGYLYIAIYPTPAITE

Nucleotide

• Length: 360 bp

>AT53522 NZ_CP011132:2837908-2838267 [Citrobacter amalonaticus Y19]
TTGTCGAAGAAAACCACGATAACAACGAATGATGTCAGCGAACCATGGTGGGGGCTCAGACGCAGCATCTCCCCGTGCTT
TGGTGCCCGTCTGGTCCAGGAGGCCAATTGCCTGCACTATCTCGCCGACCGCACCAGTATCACCGGGCAGTTCAGCGACG
CTGATTTGTGCCATCTCGATCAGGCGTTTCCACTGCTGTTGAAACAACTGGAACTCATGCTCACCAGCGGTGAACTCACT
CCCCGCTATCAGCATCGCGTCACGCTCCACGCAAAAGGACTGACCTGCGAGGCGGATACCCTCGGCAGTTGTGGTTATCT
CTATATCGCGATTTACCCGACACCTGCAATAACCGAATAA

Structures

Toxin

• AlphaFold DB: A0A0F6TVT5

Antitoxin

• AlphaFold DB: A0A0F6RFT5