Detailed information of TA system TA053512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1553528..1555113
• Replicon: chromosome
• Accession: NZ_CP011132
• Organism: Citrobacter amalonaticus Y19

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: M1KHH2
• Locus tag: F384_RS07060
• Protein ID: WP_046480828.1
• Coordinates: 1553794..1555113 (+)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: F384_RS07055
• Protein ID: WP_046480827.1
• Coordinates: 1553528..1553794 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F384_RS29805	1551940..1553340	+	1401	WP_155403983.1	autotransporter outer membrane beta-barrel domain-containing protein	-
F384_RS07055	1553528..1553794	+	267	WP_046480827.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
F384_RS07060	1553794..1555113	+	1320	WP_046480828.1	type II toxin-antitoxin system HipA family toxin	Toxin
F384_RS07065	1555424..1558618	-	3195	WP_080949898.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49019.58 Da
• Isoelectric Point: 8.1221

>T53512 WP_046480828.1 NZ_CP011132:1553794-1555113 [Citrobacter amalonaticus Y19]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPDWLANRYARPLSLSLPLQVGNITSDAVFNFFDNLLPDSPIVRDRIVKRYR
AKSKQPFDLLSEIGRDSVGAVTLLPENEAVTNPVMTWEKLSEAKLEEVLSAYKSDIPLGMINEENNFRISVAGAQEKTAL
LRMGNDWCIPKGVTPTTHIIKLPIGEIRQPNATLDLSQSVDNEYFCLLLAKQLGLDVPGAEIIKAGRVRALAVERFDRRW
NAERSVLLRLPQEDMCQTFGLPSSVKYESDGGPGIEQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGRKTAIDKIYPRHFMATAKALKFPQEQMREILCTFAEQIP
AALDNVTAALPTDFPQNVAMAIRGNVLRLHGRLNREMEE

Nucleotide

• Length: 1320 bp

>T53512 NZ_CP011132:1553794-1555113 [Citrobacter amalonaticus Y19]
ATGCCTAAACTCGTAACGTGGATGAATAATCAGCGGGTAGGCGAGTTGACGAAACTGGCCAACGGTGCGCATACATTTAA
ATATGCCCCGGATTGGTTAGCCAACCGCTATGCCAGGCCATTGTCGCTTTCGCTGCCATTGCAGGTGGGGAATATCACTT
CTGATGCCGTGTTTAACTTCTTCGATAACTTGTTGCCTGATAGCCCAATCGTGCGCGACAGGATCGTTAAACGCTATCGT
GCAAAATCAAAGCAACCTTTTGATTTATTGTCAGAAATAGGTCGAGATAGCGTGGGGGCTGTGACATTGTTACCGGAAAA
TGAAGCGGTGACAAATCCGGTGATGACATGGGAAAAGTTAAGTGAGGCAAAGCTTGAAGAGGTGCTGTCGGCTTATAAAT
CAGATATTCCGCTGGGCATGATTAATGAAGAAAATAACTTTCGTATCTCAGTTGCCGGCGCGCAGGAAAAGACCGCGCTG
CTCAGAATGGGCAATGACTGGTGTATCCCAAAAGGGGTAACGCCAACTACGCATATTATTAAGTTGCCTATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAATATTTTTGTCTACTGCTGGCGAAACAACTCG
GACTGGATGTTCCTGGAGCCGAAATTATCAAGGCGGGAAGGGTGCGTGCGTTAGCGGTCGAGCGCTTTGACAGACGCTGG
AATGCTGAACGGTCAGTTTTACTCCGCTTGCCGCAAGAAGATATGTGCCAGACATTCGGTTTACCGTCATCAGTCAAATA
CGAATCTGACGGGGGACCTGGCATCGAGCAAATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGTGCAACGGATGGCCATGCGAAAAACTTCTCTGTTTTTATTCAG
GCCGGCGGCAGTTATCGGCTCACGCCATTTTACGACATTATTTCGGCATTTCCGGTTCTTGGCGGTACGGGAATACATAT
CAGTGATTTGAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAGAAAAACAGCTATCGATAAAATTTATCCGCGTCATT
TTATGGCAACGGCAAAGGCGCTGAAATTTCCGCAAGAGCAGATGCGAGAAATTCTGTGTACGTTTGCAGAACAAATACCC
GCAGCACTGGATAACGTGACTGCCGCATTGCCAACAGATTTTCCACAGAACGTTGCGATGGCAATCAGGGGGAATGTGCT
CAGGCTGCATGGTCGGTTAAACCGGGAAATGGAGGAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9959.39 Da
• Isoelectric Point: 4.9632

>AT53512 WP_046480827.1 NZ_CP011132:1553528-1553794 [Citrobacter amalonaticus Y19]
MKSFPMIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNSTLMTFFKILQSLELSMTLCETKDAAPES
TDQQGLEW

Nucleotide

• Length: 267 bp

>AT53512 NZ_CP011132:1553528-1553794 [Citrobacter amalonaticus Y19]
ATGAAGAGCTTCCCGATGATCTACAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATAAAGCAGGCAACCATTTCCAATTTCGAGAATAACCCTGACAACAGCACAT
TGATGACCTTTTTTAAAATTTTACAGTCACTTGAGCTCTCAATGACGCTATGCGAAACAAAAGACGCGGCGCCGGAATCT
ACTGACCAACAGGGTCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: M1KHH2