53509

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 461834..462454 Replicon chromosome
Accession NZ_CP011132
Organism Citrobacter amalonaticus Y19

Toxin (Protein)


Gene name Hha Uniprot ID V1H8E6
Locus tag F384_RS02065 Protein ID WP_001280991.1
Coordinates 461834..462052 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0F6TT58
Locus tag F384_RS02070 Protein ID WP_046477029.1
Coordinates 462080..462454 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F384_RS02040 457485..458081 + 597 WP_046497614.1 membrane protein -
F384_RS02045 458163..458516 + 354 WP_046476496.1 DUF1428 family protein -
F384_RS02050 458556..460106 - 1551 WP_046476499.1 EAL domain-containing protein -
F384_RS28375 460327..460467 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
F384_RS02055 460520..460990 - 471 WP_046476502.1 membrane protein -
F384_RS02060 461105..461656 - 552 WP_046476504.1 maltose O-acetyltransferase -
F384_RS02065 461834..462052 - 219 WP_001280991.1 hemolysin expression modulator Hha Toxin
F384_RS02070 462080..462454 - 375 WP_046477029.1 Hha toxicity modulator TomB Antitoxin
F384_RS02075 462968..466117 - 3150 WP_046477031.1 multidrug efflux RND transporter permease subunit AcrB -
F384_RS02080 466140..467333 - 1194 WP_046477034.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T53509 WP_001280991.1 NZ_CP011132:c462052-461834 [Citrobacter amalonaticus Y19]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T53509 NZ_CP011132:c462052-461834 [Citrobacter amalonaticus Y19]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTGCGACGCTGTCAGACAATTGACACGCTTGAGCGCGT
GATTGAGAAAAATAAATATGAATTATCTGACAATGAACTGGCTGTATTTTACTCAGCTGCCGATCACCGTCTCGCAGAAT
TGACGATGAATAAGCTTTACGACAAGATCCCCTCCTCCGTCTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14465.28 Da        Isoelectric Point: 4.8989

>AT53509 WP_046477029.1 NZ_CP011132:c462454-462080 [Citrobacter amalonaticus Y19]
MDEYSPKRHDIAQLKFLCEALYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT53509 NZ_CP011132:c462454-462080 [Citrobacter amalonaticus Y19]
ATGGATGAATACTCACCCAAAAGACATGATATAGCGCAGCTTAAATTTCTTTGTGAAGCGCTTTATCACGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCAATCAACCTTCAACTTAACGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGAGCAGATTGATGAATATCTG
GACGACACATTTATGTTGTTCAGCAGTTATGGCATTAATGCGCAGGATCTGCAGAAATGGCGGAAATCCGGTAATCGCTT
GTTCCGCTGCTTTGTGAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ICG

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6TT58

References