Detailed information of TA system TA053506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4371565..4372183
• Replicon: chromosome
• Accession: NZ_CP011124
• Organism: Escherichia coli strain USML2

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: USML2_RS22360
• Protein ID: WP_001290581.1
• Coordinates: 4371965..4372183 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: USML2_RS22355
• Protein ID: WP_000344800.1
• Coordinates: 4371565..4371939 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
USML2_RS22345	4366654..4367847	+	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
USML2_RS22350	4367870..4371019	+	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
USML2_RS22355	4371565..4371939	+	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
USML2_RS22360	4371965..4372183	+	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
USML2_RS22365	4372355..4372906	+	552	WP_000102564.1	maltose O-acetyltransferase	-
USML2_RS22370	4373022..4373492	+	471	WP_000136192.1	YlaC family protein	-
USML2_RS22375	4373656..4375206	+	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
USML2_RS22380	4375248..4375601	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
USML2_RS22390	4375980..4376291	+	312	WP_000409911.1	MGMT family protein	-
USML2_RS22395	4376322..4376894	-	573	WP_000779842.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T53506 WP_001290581.1 NZ_CP011124:4371965-4372183 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T53506 NZ_CP011124:4371965-4372183 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT53506 WP_000344800.1 NZ_CP011124:4371565-4371939 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT53506 NZ_CP011124:4371565-4371939 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5