Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2796876..2797707 Replicon chromosome
Accession NZ_CP011124
Organism Escherichia coli strain USML2

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag USML2_RS14050 Protein ID WP_000854814.1
Coordinates 2796876..2797250 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag USML2_RS14055 Protein ID WP_001285584.1
Coordinates 2797339..2797707 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
USML2_RS14015 2792272..2793438 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
USML2_RS14020 2793557..2794030 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
USML2_RS14025 2794228..2795286 + 1059 WP_001200891.1 FUSC family protein -
USML2_RS14030 2795458..2795787 + 330 WP_000450409.1 DUF496 family protein -
USML2_RS14035 2795888..2796070 - 183 WP_001016348.1 hypothetical protein -
USML2_RS14040 2796142..2796270 + 129 Protein_2562 transposase domain-containing protein -
USML2_RS23245 2796559..2796672 - 114 WP_001161660.1 DUF957 domain-containing protein -
USML2_RS14045 2796685..2796879 - 195 WP_000988600.1 hypothetical protein -
USML2_RS14050 2796876..2797250 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
USML2_RS14055 2797339..2797707 - 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
USML2_RS14060 2797781..2798002 - 222 WP_000692323.1 DUF987 domain-containing protein -
USML2_RS14065 2798065..2798511 - 447 WP_000187523.1 RadC family protein -
USML2_RS14070 2798508..2800040 - 1533 WP_001350525.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T53494 WP_000854814.1 NZ_CP011124:c2797250-2796876 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T53494 NZ_CP011124:c2797250-2796876 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT53494 WP_001285584.1 NZ_CP011124:c2797707-2797339 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT53494 NZ_CP011124:c2797707-2797339 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References