Detailed information of TA system TA053483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 336636..337450
• Replicon: chromosome
• Accession: NZ_CP011124
• Organism: Escherichia coli strain USML2

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: USML2_RS01630
• Protein ID: WP_001054376.1
• Coordinates: 336636..336893 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: USML2_RS01635
• Protein ID: WP_001309181.1
• Coordinates: 336905..337450 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
USML2_RS01600	331924..333030	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
USML2_RS01605	333095..334075	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
USML2_RS01615	334658..335898	-	1241	Protein_299	helicase YjhR	-
USML2_RS23100	336014..336259	+	246	Protein_300	GNAT family N-acetyltransferase	-
USML2_RS01630	336636..336893	+	258	WP_001054376.1	hypothetical protein	Toxin
USML2_RS01635	336905..337450	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
USML2_RS01640	337506..338252	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
USML2_RS01645	338421..338639	+	219	Protein_304	hypothetical protein	-
USML2_RS23435	338677..338793	+	117	Protein_305	VOC family protein	-
USML2_RS01655	339038..340159	+	1122	WP_000010829.1	M42 family metallopeptidase	-
USML2_RS01660	340156..340434	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
USML2_RS01665	340446..341759	+	1314	WP_000460842.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	325646..345674	20028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T53483 WP_001054376.1 NZ_CP011124:336636-336893 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T53483 NZ_CP011124:336636-336893 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT53483 WP_001309181.1 NZ_CP011124:336905-337450 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT53483 NZ_CP011124:336905-337450 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8