Detailed information of TA system TA053476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 52312..52991
• Replicon: plasmid unnamed2
• Accession: NZ_CP011122
• Organism: Acetobacter oryzifermentans strain SLV-7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A2G4REI9
• Locus tag: WG31_RS14310
• Protein ID: WP_035362716.1
• Coordinates: 52581..52991 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: WG31_RS14305
• Protein ID: WP_003626741.1
• Coordinates: 52312..52584 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WG31_RS14265	47344..47919	-	576	WP_003626726.1	MucR family transcriptional regulator	-
WG31_RS14270	48043..48321	-	279	WP_003626728.1	hypothetical protein	-
WG31_RS14275	48763..49071	-	309	WP_035362714.1	hypothetical protein	-
WG31_RS14280	49304..49606	+	303	WP_003626733.1	hypothetical protein	-
WG31_RS15880	49599..49745	+	147	WP_020936778.1	hypothetical protein	-
WG31_RS15635	49816..49962	+	147	WP_157884547.1	hypothetical protein	-
WG31_RS14285	50031..50177	+	147	WP_003626735.1	hypothetical protein	-
WG31_RS14290	50835..51323	+	489	Protein_46	single-stranded DNA-binding protein	-
WG31_RS14295	51386..51826	+	441	WP_003626739.1	hypothetical protein	-
WG31_RS14300	51823..52239	+	417	WP_003626740.1	hypothetical protein	-
WG31_RS14305	52312..52584	+	273	WP_003626741.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
WG31_RS14310	52581..52991	+	411	WP_035362716.1	type II toxin-antitoxin system VapC family toxin	Toxin
WG31_RS14315	53128..53592	+	465	WP_020936773.1	hypothetical protein	-
WG31_RS14320	53589..53789	+	201	WP_006560470.1	hypothetical protein	-
WG31_RS14325	54379..54780	-	402	WP_020936770.1	hypothetical protein	-
WG31_RS14330	54792..55595	-	804	WP_003626746.1	hypothetical protein	-
WG31_RS14335	55692..56156	-	465	WP_020936768.1	hypothetical protein	-
WG31_RS14340	56159..57007	-	849	WP_035362718.1	hypothetical protein	-
WG31_RS14345	57125..57946	-	822	WP_035362728.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..116245	116245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-118)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14760.08 Da
• Isoelectric Point: 5.1853

>T53476 WP_035362716.1 NZ_CP011122:52581-52991 [Acetobacter oryzifermentans]
VNAYLLDTNIISDLVRNPSGPCARRVERINPADLCTSIIVAAELRYGCAKKGSAKLLNRVESVLAFVPVLPLDIPADCEY
GGIRAELEAAGQTIGMNDLLIAAHAYTLKATLVTNNTREFMCIRGLKVENWLEDGT

Nucleotide

• Length: 411 bp

>T53476 NZ_CP011122:52581-52991 [Acetobacter oryzifermentans]
GTGAACGCCTATCTCCTTGATACCAATATCATCAGTGATCTTGTCCGAAATCCTTCCGGCCCATGTGCGCGTCGCGTCGA
ACGTATCAATCCCGCCGACCTTTGTACCAGCATCATTGTGGCCGCTGAGTTACGATATGGATGTGCTAAAAAAGGCTCTG
CAAAGCTGTTAAACCGGGTTGAAAGCGTGCTGGCGTTTGTCCCCGTGCTGCCTCTCGATATTCCTGCCGACTGTGAATAC
GGTGGCATCCGCGCCGAACTCGAAGCCGCAGGGCAGACGATCGGCATGAATGATCTGCTCATCGCGGCTCACGCCTACAC
CCTCAAGGCGACCCTGGTGACGAACAACACACGTGAGTTTATGTGCATCCGAGGCTTGAAGGTTGAAAACTGGCTGGAGG
ATGGGACGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10446.07 Da
• Isoelectric Point: 4.5749

>AT53476 WP_003626741.1 NZ_CP011122:52312-52584 [Acetobacter oryzifermentans]
MPVPEMTPRPREVKLFRNNRSQAVRIPVEFEMPGDRVLIRRDGDKLVLEPVKTPSTLKELLMAWREEPQLAPEDDFPDIQ
DVAARPEDIL

Nucleotide

• Length: 273 bp

>AT53476 NZ_CP011122:52312-52584 [Acetobacter oryzifermentans]
ATGCCTGTTCCTGAAATGACTCCCCGACCGCGCGAAGTGAAACTCTTTCGGAACAATCGCAGTCAGGCTGTGCGTATTCC
GGTCGAATTTGAAATGCCTGGAGATCGCGTGTTGATCCGCCGTGACGGTGACAAGCTCGTTCTGGAGCCTGTGAAAACCC
CTTCAACGTTGAAGGAGCTTCTCATGGCATGGCGCGAAGAGCCGCAGCTGGCCCCCGAAGACGATTTTCCCGATATTCAG
GACGTAGCCGCCAGACCGGAAGATATTCTGTGA

Structures

Toxin

• AlphaFold DB: A0A2G4REI9