Detailed information of TA system TA053454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 6297284..6297897
• Replicon: chromosome
• Accession: NZ_CP011117
• Organism: Pseudomonas synxantha strain LBUM223

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: VO64_RS29500
• Protein ID: WP_046072224.1
• Coordinates: 6297646..6297897 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: VO64_RS29495
• Protein ID: WP_046072223.1
• Coordinates: 6297284..6297649 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VO64_RS29470	6292497..6293306	+	810	WP_046072218.1	ammonia-dependent NAD(+) synthetase	-
VO64_RS29475	6293457..6294029	+	573	WP_046072219.1	DUF2239 family protein	-
VO64_RS29480	6294052..6295407	+	1356	WP_046072220.1	MATE family efflux transporter	-
VO64_RS29485	6295391..6295978	-	588	WP_046072221.1	TetR/AcrR family transcriptional regulator	-
VO64_RS29490	6296111..6297283	+	1173	WP_046072222.1	MFS transporter	-
VO64_RS29495	6297284..6297649	-	366	WP_046072223.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
VO64_RS29500	6297646..6297897	-	252	WP_046072224.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9546.13 Da
• Isoelectric Point: 10.0941

>T53454 WP_046072224.1 NZ_CP011117:c6297897-6297646 [Pseudomonas synxantha]
VSKNEKLLAKLLNEHMAFTWPELVTLLRRLGYTQIEGAGSRVKFDIGDPSAMITLHRPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T53454 NZ_CP011117:c6297897-6297646 [Pseudomonas synxantha]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAATGAGCACATGGCATTTACCTGGCCCGAGCTCGTCACACTGTT
ACGACGGCTCGGATACACACAGATTGAAGGGGCCGGAAGCCGGGTCAAATTCGACATCGGCGACCCCAGTGCAATGATCA
CGTTGCACAGGCCTCACCCCGGCAACGAACTGAAGCACTACATTCGTCGCCAGATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13211.06 Da
• Isoelectric Point: 6.6273

>AT53454 WP_046072223.1 NZ_CP011117:c6297649-6297284 [Pseudomonas synxantha]
VNNQLKHNGYIGSIEASLEDNCLFGKVLFIKALVSYEGKTVAELEAAFREAVDDYLATCQALGQTPEKPCKGSFNVRVGH
DLHLAAALAATRKKVTLNDLTRQALSEFLQHHYPEPCPAIG

Nucleotide

• Length: 366 bp

>AT53454 NZ_CP011117:c6297649-6297284 [Pseudomonas synxantha]
GTGAACAACCAACTGAAACACAATGGCTATATCGGCTCTATCGAAGCCAGCCTTGAAGACAACTGCCTGTTTGGCAAGGT
CCTGTTCATCAAGGCATTAGTGAGCTACGAAGGTAAAACCGTCGCAGAACTGGAAGCCGCCTTCCGTGAAGCGGTGGACG
ATTACCTCGCGACCTGCCAGGCCCTTGGGCAAACGCCGGAGAAGCCTTGCAAAGGTTCATTCAACGTGCGCGTCGGGCAT
GACCTGCACTTGGCAGCAGCCCTGGCAGCAACCCGTAAGAAAGTGACGTTGAATGATCTGACGCGCCAAGCGTTGAGCGA
GTTTCTACAACATCACTATCCAGAGCCATGCCCGGCGATAGGCTGA