Detailed information of TA system TA053450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/-
• Location: 22603..23209
• Replicon: chromosome
• Accession: NZ_CP011117
• Organism: Pseudomonas synxantha strain LBUM223

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: VO64_RS00120
• Protein ID: WP_046067907.1
• Coordinates: 22603..22947 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: -
• Locus tag: VO64_RS00125
• Protein ID: WP_046067908.1
• Coordinates: 22961..23209 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VO64_RS00095	18339..18524	+	186	Protein_17	transposase domain-containing protein	-
VO64_RS00100	18818..19726	-	909	WP_046067904.1	hypothetical protein	-
VO64_RS00105	19930..20910	-	981	Protein_19	IS5 family transposase	-
VO64_RS00110	20998..21401	-	404	Protein_20	transposase	-
VO64_RS00120	22603..22947	-	345	WP_046067907.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VO64_RS00125	22961..23209	-	249	WP_046067908.1	prevent-host-death protein	Antitoxin
VO64_RS00130	23451..24554	-	1104	WP_046067909.1	IS5 family transposase	-
VO64_RS00135	24682..25659	+	978	WP_046072428.1	IS5 family transposase	-
VO64_RS00140	26069..26296	-	228	WP_046072429.1	hypothetical protein	-
VO64_RS00145	26510..26731	-	222	WP_046072430.1	hypothetical protein	-
VO64_RS00150	26847..27761	-	915	WP_046067910.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	4320..39889	35569

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-99)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12953.81 Da
• Isoelectric Point: 5.9545

>T53450 WP_046067907.1 NZ_CP011117:c22947-22603 [Pseudomonas synxantha]
MTTLTIRYTNIAQQSIEDQIEHLAGFHGLGHAVDRLDTVINTLQYKLLSAPLAYPISPQASDLGILHYRELNTDGYRVFY
ETIEADQAIVVLLLLRNKQSVENALIRYCLLHTQ

Nucleotide

• Length: 345 bp

>T53450 NZ_CP011117:c22947-22603 [Pseudomonas synxantha]
ATGACAACACTAACAATCCGCTACACCAACATTGCACAACAGAGTATCGAAGACCAGATCGAGCATCTGGCAGGCTTTCA
TGGCCTGGGCCATGCCGTTGATCGGTTAGACACTGTGATTAACACCCTTCAATACAAGCTACTGTCCGCTCCATTAGCCT
ATCCAATCAGCCCTCAAGCCAGCGATCTGGGTATCCTGCACTACCGCGAACTCAATACCGATGGGTACCGAGTGTTCTAT
GAAACCATAGAAGCGGACCAGGCAATCGTCGTTCTTTTGCTGCTGCGGAATAAACAAAGCGTAGAAAATGCACTGATTCG
ATACTGTCTACTGCATACGCAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9377.78 Da
• Isoelectric Point: 9.4828

>AT53450 WP_046067908.1 NZ_CP011117:c23209-22961 [Pseudomonas synxantha]
MRVETVSFLKKYASDLPLEEPMVVTQNGVPAYVVESYAERKSRDETIALVKLLAMSSREYSQGKHCSTDELKARLSKRFA
HR

Nucleotide

• Length: 249 bp

>AT53450 NZ_CP011117:c23209-22961 [Pseudomonas synxantha]
ATGCGAGTAGAAACAGTTAGCTTTCTAAAGAAATACGCATCCGACCTGCCGCTGGAAGAGCCGATGGTCGTCACGCAAAA
CGGTGTGCCTGCATACGTTGTGGAATCTTACGCTGAGCGTAAGAGCCGCGATGAAACGATAGCCCTAGTGAAACTGCTCG
CAATGAGTTCTCGCGAGTATTCCCAAGGCAAACATTGTTCGACTGATGAACTGAAAGCCCGCTTGAGCAAGAGGTTTGCC
CACAGATAA