53428

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1348445..1349361 Replicon chromosome
Accession NZ_CP011115
Organism Bacillus subtilis KCTC 1028 = ATCC 6051a strain KCTC 1028

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag O7A_RS07160 Protein ID WP_003244695.1
Coordinates 1348615..1349361 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag O7A_RS07155 Protein ID WP_003232646.1
Coordinates 1348445..1348615 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O7A_RS07125 1345308..1345637 + 330 WP_003232660.1 phage-like element PBSX protein XkdW -
O7A_RS07130 1345634..1345798 + 165 WP_003232658.1 XkdX family protein -
O7A_RS07135 1345842..1346681 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
O7A_RS07140 1346734..1347003 + 270 WP_003232655.1 hemolysin XhlA family protein -
O7A_RS07145 1347016..1347279 + 264 WP_003232653.1 phage holin -
O7A_RS07150 1347292..1348185 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
O7A_RS23345 1348222..1348359 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
O7A_RS07155 1348445..1348615 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
O7A_RS07160 1348615..1349361 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
O7A_RS07165 1349471..1350472 - 1002 WP_003232642.1 inorganic phosphate transporter -
O7A_RS07170 1350485..1351102 - 618 WP_003218470.1 DUF47 domain-containing protein -
O7A_RS07175 1351378..1352694 - 1317 WP_003244819.1 serine/threonine exchanger -
O7A_RS07180 1353083..1354033 + 951 WP_003245086.1 ring-cleaving dioxygenase -
O7A_RS23350 1354134..1354280 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-238)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T53428 WP_003244695.1 NZ_CP011115:c1349361-1348615 [Bacillus subtilis KCTC 1028 = ATCC 6051a]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T53428 NZ_CP011115:c1349361-1348615 [Bacillus subtilis KCTC 1028 = ATCC 6051a]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT53428 WP_003232646.1 NZ_CP011115:c1348615-1348445 [Bacillus subtilis KCTC 1028 = ATCC 6051a]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT53428 NZ_CP011115:c1348615-1348445 [Bacillus subtilis KCTC 1028 = ATCC 6051a]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 3O6Q


Antitoxin

Source ID Structure
AlphaFold DB G4EXD8

References