Detailed information of TA system TA053426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 609669..610274
• Replicon: chromosome
• Accession: NZ_CP011114
• Organism: Paenibacillus durus ATCC 35681

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0F7CHC6
• Locus tag: VK70_RS02720
• Protein ID: WP_025691534.1
• Coordinates: 609924..610274 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: VK70_RS02715
• Protein ID: WP_175491834.1
• Coordinates: 609669..609920 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VK70_RS02690	605108..605320	-	213	WP_025694815.1	DUF86 domain-containing protein	-
VK70_RS02700	606116..606577	+	462	WP_025694817.1	hypothetical protein	-
VK70_RS02705	606928..608016	+	1089	WP_025694818.1	outer membrane lipoprotein carrier protein LolA	-
VK70_RS02710	608242..609444	+	1203	WP_025694819.1	alanine racemase	-
VK70_RS02715	609669..609920	+	252	WP_175491834.1	ribbon-helix-helix protein, CopG family	Antitoxin
VK70_RS02720	609924..610274	+	351	WP_025691534.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
VK70_RS02725	610477..612666	+	2190	WP_025694820.1	RNA-binding transcriptional accessory protein	-
VK70_RS02730	612814..613785	-	972	WP_025695199.1	S-layer homology domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-35)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12796.68 Da
• Isoelectric Point: 5.1171

>T53426 WP_025691534.1 NZ_CP011114:609924-610274 [Paenibacillus durus ATCC 35681]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDASSHGFDRDSVILLEQV
RTIDKQRLTDKITHLDDETMKKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T53426 NZ_CP011114:609924-610274 [Paenibacillus durus ATCC 35681]
TTGATCGTAAAACGCGGCGATGTTTTCTTTGCCGATCTTTCGCCGGTAGTTGGTTCCGAGCAGGGCGGGGTAAGGCCTGT
TCTCGTCATTCAGAACGATATTGGCAATCGCTTTAGTCCAACGGTAATTGTTGCGGCAATTACCGCACAGATCCAGAAGG
CCAAGCTGCCTACTCATGTGGAAATTGACGCGTCATCCCACGGCTTTGACCGGGATTCCGTCATTCTGCTGGAGCAGGTT
CGGACGATCGACAAGCAGCGCTTAACGGATAAGATCACCCATCTTGATGACGAAACGATGAAAAAGGTGGATGACTCGCT
GCAGATCAGTCTGGGTCTGATTGATTTTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9505.98 Da
• Isoelectric Point: 5.1184

>AT53426 WP_175491834.1 NZ_CP011114:609669-609920 [Paenibacillus durus ATCC 35681]
MISLPDHLLQEVDGIVQMENSNRSELIRQAMKLYLSERRKRSIRESMQRGYMEMAKINLTMACEAFLAEEDADSTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT53426 NZ_CP011114:609669-609920 [Paenibacillus durus ATCC 35681]
ATGATCAGCTTGCCCGATCATCTCTTGCAGGAGGTGGACGGAATTGTCCAGATGGAGAATTCCAATCGCAGTGAATTGAT
TCGGCAAGCCATGAAGCTGTATCTCAGCGAACGGAGGAAGCGTTCCATAAGGGAGTCTATGCAGCGCGGATACATGGAAA
TGGCTAAGATCAACTTGACCATGGCATGTGAAGCGTTCCTTGCCGAGGAAGATGCAGACAGTACTCTTGGCCGCTTAGTA
AGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A0F7CHC6