Detailed information of TA system TA053425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4489551..4490365
• Replicon: chromosome
• Accession: NZ_CP011113
• Organism: Escherichia coli strain RR1

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: SR35_RS23715
• Protein ID: WP_001054376.1
• Coordinates: 4490108..4490365 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: SR35_RS23710
• Protein ID: WP_001309181.1
• Coordinates: 4489551..4490096 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SR35_RS23685	4485242..4486555	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
SR35_RS23690	4486567..4486845	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
SR35_RS23695	4486842..4487963	-	1122	WP_000010829.1	M42 family metallopeptidase	-
SR35_RS25375	4488208..4488324	-	117	Protein_4210	VOC family protein	-
SR35_RS24650	4488362..4488580	-	219	Protein_4211	hypothetical protein	-
SR35_RS23705	4488749..4489495	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
SR35_RS23710	4489551..4490096	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
SR35_RS23715	4490108..4490365	-	258	WP_001054376.1	hypothetical protein	Toxin
SR35_RS25090	4490742..4490987	-	246	Protein_4215	GNAT family N-acetyltransferase	-
SR35_RS23725	4491103..4492343	+	1241	Protein_4216	helicase YjhR	-
SR35_RS23735	4492926..4493906	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
SR35_RS23740	4493971..4495077	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T53425 WP_001054376.1 NZ_CP011113:c4490365-4490108 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T53425 NZ_CP011113:c4490365-4490108 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT53425 WP_001309181.1 NZ_CP011113:c4490096-4489551 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT53425 NZ_CP011113:c4490096-4489551 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8