Detailed information of TA system TA053395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 4607373..4607917
• Replicon: chromosome
• Accession: NZ_CP011112
• Organism: Luteipulveratus mongoliensis strain MN07-A0370

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0K1JMU4
• Locus tag: VV02_RS21885
• Protein ID: WP_052595063.1
• Coordinates: 4607373..4607675 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: VV02_RS21890
• Protein ID: WP_052595065.1
• Coordinates: 4607675..4607917 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VV02_RS21855	4603098..4604054	+	957	WP_052595052.1	methionine ABC transporter substrate-binding protein	-
VV02_RS21860	4604051..4605076	+	1026	WP_052595054.1	methionine ABC transporter ATP-binding protein	-
VV02_RS21865	4605136..4605735	+	600	WP_179945388.1	ABC transporter permease	-
VV02_RS21870	4605732..4605920	-	189	WP_052595058.1	hypothetical protein	-
VV02_RS21875	4606070..4606816	+	747	WP_052595059.1	SDR family oxidoreductase	-
VV02_RS21880	4606861..4607367	+	507	WP_052595061.1	DUF664 domain-containing protein	-
VV02_RS21885	4607373..4607675	-	303	WP_052595063.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
VV02_RS21890	4607675..4607917	-	243	WP_052595065.1	ribbon-helix-helix protein, CopG family	Antitoxin
VV02_RS21895	4608024..4608326	+	303	WP_052595066.1	urease subunit gamma	-
VV02_RS21900	4608338..4608664	+	327	WP_052595069.1	urease subunit beta	-
VV02_RS21905	4608657..4610360	+	1704	WP_052595071.1	urease subunit alpha	-
VV02_RS21910	4610362..4611012	+	651	WP_052595073.1	hypothetical protein	-
VV02_RS21915	4610978..4611706	+	729	WP_083450349.1	urease accessory protein UreG	-
VV02_RS21920	4611703..4612404	+	702	WP_169787735.1	urease accessory protein UreD	-
VV02_RS21925	4612504..4612713	+	210	WP_052595079.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-40)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10956.60 Da
• Isoelectric Point: 6.2150

>T53395 WP_052595063.1 NZ_CP011112:c4607675-4607373 [Luteipulveratus mongoliensis]
MRPIHIGRTDKSRPVLVLTRELARPLLHWVTVAPITSTIRGISTEVRVGPANGLDHDCVVSCDNISTIRATDLGREVGYL
LREQEAELNAAIAAAFDLEL

Nucleotide

• Length: 303 bp

>T53395 NZ_CP011112:c4607675-4607373 [Luteipulveratus mongoliensis]
ATGCGTCCGATCCACATCGGGCGCACGGACAAGAGTCGACCTGTGCTCGTGCTCACCCGTGAGCTCGCGCGACCCCTGCT
GCACTGGGTCACGGTCGCTCCGATCACCAGCACCATCCGAGGCATCTCCACGGAGGTGAGGGTCGGCCCGGCCAACGGAC
TCGATCATGACTGCGTCGTCAGCTGCGACAACATCAGCACCATTCGGGCCACGGACCTCGGCCGCGAGGTCGGATATCTA
CTTCGAGAGCAGGAAGCCGAGCTGAACGCCGCGATCGCAGCAGCCTTCGATCTGGAGCTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8955.97 Da
• Isoelectric Point: 4.3161

>AT53395 WP_052595065.1 NZ_CP011112:c4607917-4607675 [Luteipulveratus mongoliensis]
MSTQIAVRLPDDQVAFIDEMVQRGDASSRADVVKRALDRERRRQQTLRDVEILRAAGGNPYPDLDGWLTEAAGTPMDDLD

Nucleotide

• Length: 243 bp

>AT53395 NZ_CP011112:c4607917-4607675 [Luteipulveratus mongoliensis]
ATGAGTACTCAGATCGCCGTGCGTCTTCCCGACGACCAGGTGGCGTTCATCGATGAGATGGTCCAACGTGGAGACGCGAG
TAGTCGCGCCGATGTCGTGAAGCGCGCCCTCGACCGTGAGCGACGTCGGCAGCAGACTCTGCGCGATGTGGAGATCCTGC
GAGCGGCGGGTGGCAATCCCTACCCGGACCTCGACGGCTGGCTGACCGAAGCCGCGGGCACACCGATGGACGACCTGGAC
TGA

Structures

Toxin

• AlphaFold DB: A0A0K1JMU4