Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 6320843..6321456 Replicon chromosome
Accession NZ_CP011110
Organism Pseudomonas chlororaphis strain PCL1606

Toxin (Protein)


Gene name hicA Uniprot ID A0A0C5ED99
Locus tag PCL1606_RS28340 Protein ID WP_044460628.1
Coordinates 6321274..6321456 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0C5EE99
Locus tag PCL1606_RS28335 Protein ID WP_044465918.1
Coordinates 6320843..6321244 (-) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCL1606_RS28320 6315922..6317274 - 1353 WP_045886400.1 LLM class flavin-dependent oxidoreductase -
PCL1606_RS28325 6317321..6319579 - 2259 WP_045886402.1 TonB-dependent receptor -
PCL1606_RS28330 6319787..6320833 + 1047 WP_044460629.1 LacI family DNA-binding transcriptional regulator -
PCL1606_RS28335 6320843..6321244 - 402 WP_044465918.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
PCL1606_RS28340 6321274..6321456 - 183 WP_044460628.1 type II toxin-antitoxin system HicA family toxin Toxin
PCL1606_RS28345 6321585..6322475 - 891 WP_045886404.1 LysR family transcriptional regulator -
PCL1606_RS28350 6322615..6323355 + 741 WP_045886406.1 SDR family oxidoreductase -
PCL1606_RS28355 6323515..6323772 + 258 WP_045886408.1 hypothetical protein -
PCL1606_RS28360 6323877..6325841 - 1965 WP_171820145.1 choline transporter BetT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 7018.16 Da        Isoelectric Point: 10.0476

>T53393 WP_044460628.1 NZ_CP011110:c6321456-6321274 [Pseudomonas chlororaphis]
MRSRELIRMIEEDGWYLIAVKGSHHQYRHQYKPGRVTVPHPEADLPRGTIHCILKQAGLK

Download         Length: 183 bp

>T53393 NZ_CP011110:c6321456-6321274 [Pseudomonas chlororaphis]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAGGGCAGCCATCACCAATA
CAGGCATCAGTACAAACCGGGGCGGGTTACCGTGCCTCACCCGGAGGCGGATCTGCCCCGGGGCACGATCCACTGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14717.53 Da        Isoelectric Point: 4.6438

>AT53393 WP_044465918.1 NZ_CP011110:c6321244-6320843 [Pseudomonas chlororaphis]
MKFPVVLHKDADSEYGVIVPDVPGCFSAGRTAAQAFENVKEALSLHYEGLVADGEPLPQVHEIDEHIDNPDYAGGVWGVV
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Download         Length: 402 bp

>AT53393 NZ_CP011110:c6321244-6320843 [Pseudomonas chlororaphis]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGAATACGGCGTGATTGTCCCTGATGTGCCGGGCTGTTTTTC
CGCGGGGCGCACTGCGGCGCAAGCCTTCGAGAACGTGAAGGAAGCTCTGTCGTTGCACTACGAAGGCCTGGTTGCCGATG
GCGAGCCGCTGCCCCAGGTGCACGAGATCGACGAGCATATCGACAATCCCGACTATGCCGGCGGAGTGTGGGGCGTGGTC
GAGTTCGACATCACGCCGTACTTCGGCAAGGCCGTGCGGTTCAACGCGACATTGCCGGAGCAACTGCTGGAGCGCATCGA
CCAGACGGTCAAGCGCGACCAGCGCTATCGCTCGCGTTCGGGCTTTCTCGCCGCGGCGGCATTGCGCGAATTGTCCGCGT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C5ED99

Antitoxin

Source ID Structure
AlphaFold DB A0A0C5EE99

References