Detailed information of TA system TA053384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1312372..1313000
• Replicon: chromosome
• Accession: NZ_CP011110
• Organism: Pseudomonas chlororaphis strain PCL1606

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: PCL1606_RS06185
• Protein ID: WP_045881460.1
• Coordinates: 1312602..1313000 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: PCL1606_RS06180
• Protein ID: WP_045881459.1
• Coordinates: 1312372..1312602 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCL1606_RS06170	1307766..1311176	-	3411	WP_045881457.1	type I-F CRISPR-associated helicase Cas3	-
PCL1606_RS06175	1311173..1312150	-	978	WP_044464870.1	type I-F CRISPR-associated endonuclease Cas1	-
PCL1606_RS06180	1312372..1312602	+	231	WP_045881459.1	antitoxin	Antitoxin
PCL1606_RS06185	1312602..1313000	+	399	WP_045881460.1	tRNA(fMet)-specific endonuclease VapC	Toxin
PCL1606_RS06190	1313040..1314974	-	1935	WP_045881461.1	alkaline phosphatase family protein	-
PCL1606_RS06195	1315075..1315683	-	609	WP_044464867.1	LysE family translocator	-
PCL1606_RS06200	1315971..1316900	-	930	WP_045881462.1	transcriptional regulator GcvA	-
PCL1606_RS06205	1317036..1317566	-	531	WP_044464865.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14878.17 Da
• Isoelectric Point: 7.3226

>T53384 WP_045881460.1 NZ_CP011110:1312602-1313000 [Pseudomonas chlororaphis]
MLKYMLDTNICIFTIKNKPQEVREAFNRQHGRLCISSVTLMELMYGAEKSAAPERNLSVIEGFVARLEVLAYDHEAAIHS
GQLRAELARAGTPIGPYDQLIAGHARSRGLVLVTNNVREFERVPGLRIEDWL

Nucleotide

• Length: 399 bp

>T53384 NZ_CP011110:1312602-1313000 [Pseudomonas chlororaphis]
ATGCTTAAGTACATGCTTGACACGAACATCTGTATTTTCACGATCAAGAACAAACCCCAGGAAGTTCGAGAAGCGTTCAA
TCGCCAGCACGGCCGGTTGTGTATCAGCTCAGTGACGTTGATGGAATTGATGTATGGCGCTGAGAAGTCTGCGGCCCCCG
AGCGAAACCTATCGGTCATCGAGGGGTTCGTGGCCCGCCTTGAAGTGCTCGCTTATGACCATGAAGCCGCTATCCACAGT
GGCCAGCTGCGTGCCGAGCTGGCCAGGGCAGGCACGCCAATTGGCCCTTACGATCAATTGATTGCAGGTCATGCCAGGTC
CCGGGGATTAGTACTGGTGACCAACAATGTGCGTGAGTTCGAGCGAGTGCCTGGGCTGCGCATTGAGGACTGGCTCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8555.55 Da
• Isoelectric Point: 4.1963

>AT53384 WP_045881459.1 NZ_CP011110:1312372-1312602 [Pseudomonas chlororaphis]
MEQGSVFKSNRSQAIRMPKAVALPDDVTRVDIVAIGRARIITPAGEAWDSWFDDEPVSADFMCERDQPAEQEREAF

Nucleotide

• Length: 231 bp

>AT53384 NZ_CP011110:1312372-1312602 [Pseudomonas chlororaphis]
ATGGAGCAAGGATCGGTATTCAAAAGCAATCGTAGTCAGGCTATCCGAATGCCCAAGGCGGTGGCACTCCCTGATGATGT
GACTCGGGTAGATATCGTTGCGATAGGACGCGCCCGGATCATTACCCCAGCAGGCGAGGCCTGGGACAGTTGGTTCGATG
ACGAGCCAGTGAGCGCGGATTTCATGTGTGAACGTGATCAGCCGGCAGAACAAGAACGTGAGGCGTTCTGA