Detailed information of TA system TA053366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 3873903..3874485
• Replicon: chromosome
• Accession: NZ_CP011104
• Organism: Photorhabdus thracensis strain DSM 15199

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A081RUW7
• Locus tag: VY86_RS16675
• Protein ID: WP_036840095.1
• Coordinates: 3874189..3874485 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: VY86_RS16670
• Protein ID: WP_036840097.1
• Coordinates: 3873903..3874187 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VY86_RS23640	3869109..3869345	+	237	WP_081858331.1	tail fiber assembly protein	-
VY86_RS16655	3869386..3870483	-	1098	WP_036840099.1	glycosyltransferase family 4 protein	-
VY86_RS16660	3871464..3872090	-	627	WP_036840102.1	tail fiber assembly protein	-
VY86_RS24230	3872090..3872920	-	831	WP_081858332.1	phage tail protein	-
VY86_RS16670	3873903..3874187	-	285	WP_036840097.1	putative addiction module antidote protein	Antitoxin
VY86_RS16675	3874189..3874485	-	297	WP_036840095.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VY86_RS23645	3874605..3874840	-	236	Protein_3313	transposase	-
VY86_RS16680	3875027..3876538	-	1512	WP_036840092.1	hypothetical protein	-
VY86_RS16685	3876962..3877558	-	597	WP_036840090.1	Tat proofreading chaperone DmsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3858614..3874485	15871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-91)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11288.07 Da
• Isoelectric Point: 10.3896

>T53366 WP_036840095.1 NZ_CP011104:c3874485-3874189 [Photorhabdus thracensis]
MIKVNRTPEVERWLKSLKDKTAKAKIIIRIDRMKEGNFGDAEPVGNGISELRIHQGKGYRVYFTNRNNEIVLLLCGGDKN
TQQQDIKKAKEIAKEWGF

Nucleotide

• Length: 297 bp

>T53366 NZ_CP011104:c3874485-3874189 [Photorhabdus thracensis]
ATGATTAAAGTAAATCGAACACCCGAAGTAGAGAGATGGTTAAAATCTTTGAAAGACAAAACCGCAAAAGCAAAAATCAT
TATAAGAATTGATAGAATGAAAGAGGGTAATTTCGGAGATGCAGAACCTGTTGGAAATGGAATATCAGAATTAAGAATTC
ATCAAGGGAAGGGATACAGGGTATATTTTACTAATAGGAATAATGAAATAGTTTTGTTGCTTTGTGGTGGCGATAAAAAT
ACACAACAACAAGATATCAAAAAAGCAAAAGAAATAGCTAAAGAATGGGGATTTTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10508.99 Da
• Isoelectric Point: 5.6938

>AT53366 WP_036840097.1 NZ_CP011104:c3874187-3873903 [Photorhabdus thracensis]
MNKELKEFDVVEFLHDDEDIQAYLNAAIEENDTKYLFIALGNIARAKNISQLSKQVGMSREGIYKALSGEANPTFNTVFK
ITKALGLKLHFTGA

Nucleotide

• Length: 285 bp

>AT53366 NZ_CP011104:c3874187-3873903 [Photorhabdus thracensis]
ATGAATAAAGAGCTTAAAGAGTTTGATGTTGTTGAGTTTTTACATGATGATGAAGATATTCAAGCCTATTTAAATGCAGC
GATAGAGGAAAACGATACTAAATATTTATTTATTGCATTAGGCAATATTGCAAGAGCAAAAAATATCAGCCAGCTATCAA
AACAAGTTGGAATGAGTAGAGAGGGTATTTATAAAGCACTTTCTGGAGAAGCAAACCCTACATTTAATACAGTGTTTAAA
ATAACGAAAGCGTTGGGTTTAAAATTACATTTCACAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A081RUW7