Detailed information of TA system TA053364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3810019..3810805
• Replicon: chromosome
• Accession: NZ_CP011104
• Organism: Photorhabdus thracensis strain DSM 15199

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: VY86_RS16355
• Protein ID: WP_158020039.1
• Coordinates: 3810019..3810519 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: T0PA38
• Locus tag: VY86_RS16360
• Protein ID: WP_021325292.1
• Coordinates: 3810527..3810805 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VY86_RS16345	3808259..3808765	+	507	WP_046975758.1	CdiI family contact-dependent growth inhibition immunity protein	-
VY86_RS16350	3808919..3809641	-	723	WP_031282008.1	PAS and helix-turn-helix domain-containing protein	-
VY86_RS16355	3810019..3810519	-	501	WP_158020039.1	GNAT family N-acetyltransferase	Toxin
VY86_RS16360	3810527..3810805	-	279	WP_021325292.1	DUF1778 domain-containing protein	Antitoxin
VY86_RS16365	3811085..3812497	+	1413	Protein_3251	DUF637 domain-containing protein	-
VY86_RS16370	3812497..3812997	+	501	WP_046975761.1	CdiI family contact-dependent growth inhibition immunity protein	-
VY86_RS24220	3813158..3813547	+	390	Protein_3253	CdiI family contact-dependent growth inhibition immunity protein	-
VY86_RS16375	3813884..3814612	-	729	WP_046976925.1	PAS and helix-turn-helix domain-containing protein	-
VY86_RS16380	3814910..3815194	-	285	WP_046975762.1	helix-turn-helix domain-containing protein	-
VY86_RS16385	3815196..3815528	-	333	WP_023046557.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3808919..3817788	8869

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-92)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18182.85 Da
• Isoelectric Point: 9.7646

>T53364 WP_158020039.1 NZ_CP011104:c3810519-3810019 [Photorhabdus thracensis]
MSNGLTPLTDDHNLDNFNSGVDTLDNWLINKASKNQKGNASRTYVVSEDKNVIAYYSLATGSIMSQEAISKFKRNMPNPI
PVIILGRLAVDKNYQGKGFGRYLVRDAAIRVTQAADLVGIRGLIVHAISEDAKAFYKSVGFDESPLNPMTLMITLKDLKT
AFGIKG

Nucleotide

• Length: 501 bp

>T53364 NZ_CP011104:c3810519-3810019 [Photorhabdus thracensis]
ATGAGTAATGGCCTAACCCCATTAACAGACGATCACAATTTAGATAATTTTAATTCAGGAGTTGACACCCTTGACAATTG
GTTAATAAACAAAGCATCAAAAAACCAAAAAGGAAATGCCTCACGAACATACGTTGTAAGCGAAGACAAAAATGTTATTG
CCTATTATTCGTTGGCTACCGGCTCAATTATGTCGCAAGAAGCGATAAGCAAATTTAAACGCAATATGCCGAACCCCATT
CCGGTAATCATTTTGGGCCGTCTGGCTGTTGATAAAAATTATCAAGGAAAAGGGTTTGGCCGCTATTTGGTTAGAGATGC
TGCCATACGCGTCACACAAGCCGCAGATTTAGTTGGTATTAGAGGATTAATTGTCCATGCTATCTCAGAAGACGCTAAAG
CATTTTATAAATCTGTGGGTTTTGATGAATCGCCTTTAAACCCAATGACGTTGATGATCACATTAAAGGATTTAAAGACC
GCTTTCGGAATCAAAGGTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10626.26 Da
• Isoelectric Point: 9.6880

>AT53364 WP_021325292.1 NZ_CP011104:c3810805-3810527 [Photorhabdus thracensis]
MNVTIENRKNKTLNLRIRQEDRDLIDRAAKVKGKTVTEYVLDTIKRDAENTLLEHSFMVVSPEVFDAFVAKLDDPAAPNE
RLIKTANMKKPW

Nucleotide

• Length: 279 bp

>AT53364 NZ_CP011104:c3810805-3810527 [Photorhabdus thracensis]
ATGAATGTAACAATTGAGAATCGTAAAAATAAGACACTCAACTTGAGGATTAGGCAAGAAGATCGAGATTTAATCGATAG
GGCAGCAAAAGTAAAAGGCAAAACCGTCACCGAATATGTCCTTGATACAATCAAACGTGATGCTGAAAACACCCTATTGG
AACACTCATTTATGGTTGTTTCTCCAGAAGTTTTTGATGCTTTTGTCGCTAAACTTGATGATCCGGCAGCACCAAATGAA
CGTCTTATCAAAACAGCAAATATGAAAAAACCTTGGTGA

Structures

Antitoxin

• AlphaFold DB: T0PA38