Detailed information of TA system TA053362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 3205351..3205968
• Replicon: chromosome
• Accession: NZ_CP011104
• Organism: Photorhabdus thracensis strain DSM 15199

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A329WUQ3
• Locus tag: VY86_RS13885
• Protein ID: WP_046976882.1
• Coordinates: 3205351..3205647 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: VY86_RS13890
• Protein ID: WP_046975380.1
• Coordinates: 3205651..3205968 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VY86_RS13860	3200739..3201161	+	423	WP_046975375.1	regulatory protein GemA	-
VY86_RS13865	3201154..3201522	+	369	WP_046975376.1	DNA-binding protein	-
VY86_RS13870	3201743..3203377	-	1635	WP_167630902.1	PilN family type IVB pilus formation outer membrane protein	-
VY86_RS13875	3203462..3203899	-	438	WP_046975378.1	type IV pilus biogenesis protein PilM	-
VY86_RS13880	3203906..3204955	-	1050	WP_046975379.1	TcpQ domain-containing protein	-
VY86_RS13885	3205351..3205647	+	297	WP_046976882.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VY86_RS13890	3205651..3205968	+	318	WP_046975380.1	putative addiction module antidote protein	Antitoxin
VY86_RS13895	3206401..3207510	-	1110	WP_046973751.1	ISAs1 family transposase	-
VY86_RS13905	3207932..3210778	-	2847	WP_046975381.1	toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3206401..3207510	1109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11130.98 Da
• Isoelectric Point: 10.3368

>T53362 WP_046976882.1 NZ_CP011104:3205351-3205647 [Photorhabdus thracensis]
MIELIKTDFFDRWLRNLRDARAKARIETRIRRLSLGNPGDVKPVGEGISEMRIDYGPGYRVYFIAKGPIVIVLLCGGDKG
SQSHDIKLAKTIATQWKE

Nucleotide

• Length: 297 bp

>T53362 NZ_CP011104:3205351-3205647 [Photorhabdus thracensis]
ATGATAGAACTCATCAAAACAGACTTTTTTGACCGCTGGCTTCGTAATCTTCGTGATGCACGGGCCAAAGCGAGGATCGA
AACACGAATCAGGCGCTTGAGCCTCGGAAATCCAGGTGATGTGAAACCTGTAGGTGAAGGTATTTCAGAAATGAGAATAG
ACTATGGCCCAGGATACAGAGTCTACTTCATAGCTAAAGGCCCCATTGTCATTGTGCTGCTGTGTGGTGGAGACAAAGGC
AGCCAATCCCACGATATTAAATTGGCAAAAACCATTGCCACGCAATGGAAGGAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11442.91 Da
• Isoelectric Point: 5.0774

>AT53362 WP_046975380.1 NZ_CP011104:3205651-3205968 [Photorhabdus thracensis]
MKSQKVKFSRYDTADYLQTEEDIAAYLEAVMEDGDPSLIAAALGDIARARNISQLARDVGMSREGLYKALSGEGNPTFTT
ITKVANALGLRFTMQPISTPTTHTQ

Nucleotide

• Length: 318 bp

>AT53362 NZ_CP011104:3205651-3205968 [Photorhabdus thracensis]
ATGAAAAGCCAAAAAGTAAAGTTCAGCCGCTACGATACTGCAGACTATCTCCAAACAGAAGAAGATATTGCGGCATATCT
GGAGGCCGTAATGGAAGATGGTGATCCTTCCCTTATTGCGGCTGCTTTGGGTGACATAGCACGAGCCCGTAACATCAGCC
AACTAGCAAGAGATGTTGGAATGAGCCGAGAAGGGCTTTACAAGGCCCTTTCTGGCGAGGGAAACCCTACATTTACGACA
ATCACAAAAGTTGCTAACGCCTTAGGACTACGTTTTACTATGCAACCCATTAGTACACCGACAACGCACACTCAATAG

Structures

Toxin

• AlphaFold DB: A0A329WUQ3