Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 280064..280640 Replicon chromosome
Accession NZ_CP011104
Organism Photorhabdus thracensis strain DSM 15199

Toxin (Protein)


Gene name shpA Uniprot ID A0A0F7LJ90
Locus tag VY86_RS00925 Protein ID WP_046973601.1
Coordinates 280353..280640 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID T0NTR3
Locus tag VY86_RS00920 Protein ID WP_021326460.1
Coordinates 280064..280366 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VY86_RS00905 276197..277198 + 1002 WP_046973598.1 LacI family DNA-binding transcriptional regulator -
VY86_RS00910 277349..278803 + 1455 WP_046973599.1 Na+/H+ antiporter NhaC -
VY86_RS00915 278790..279968 + 1179 WP_046973600.1 pyridoxal phosphate-dependent aminotransferase -
VY86_RS00920 280064..280366 - 303 WP_021326460.1 BrnA antitoxin family protein Antitoxin
VY86_RS00925 280353..280640 - 288 WP_046973601.1 BrnT family toxin Toxin
VY86_RS00930 281304..281516 - 213 WP_046973602.1 helix-turn-helix domain-containing protein -
VY86_RS00935 282526..283215 + 690 WP_046973603.1 PAS domain-containing protein -
VY86_RS00940 283257..283943 + 687 WP_046973604.1 PAS and helix-turn-helix domain-containing protein -
VY86_RS00945 284161..284847 + 687 WP_046973605.1 PAS and helix-turn-helix domain-containing protein -
VY86_RS24370 284986..285160 + 175 Protein_179 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 261439..298123 36684


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(12-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11283.72 Da        Isoelectric Point: 6.0921

>T53351 WP_046973601.1 NZ_CP011104:c280640-280353 [Photorhabdus thracensis]
MELGFEWDLTKAETNFRKHGIRFEEAALVFDDPFHLSLQDRFENGEYRWQTIGTVKECVVVLVAHTIRFEDGTEVIRIIS
ARKAERNERKRYEHG

Download         Length: 288 bp

>T53351 NZ_CP011104:c280640-280353 [Photorhabdus thracensis]
ATGGAGCTTGGGTTTGAATGGGACCTCACTAAAGCGGAAACAAATTTCCGTAAGCACGGGATTCGTTTTGAAGAAGCGGC
TCTTGTTTTTGATGACCCTTTTCATCTGTCATTGCAGGATCGTTTTGAAAATGGTGAATATCGCTGGCAGACGATTGGAA
CAGTGAAAGAATGTGTGGTTGTTCTGGTCGCTCATACGATACGGTTTGAAGATGGTACCGAGGTTATTCGTATTATCAGT
GCACGAAAAGCAGAGCGGAATGAGAGGAAACGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11629.44 Da        Isoelectric Point: 10.2782

>AT53351 WP_021326460.1 NZ_CP011104:c280366-280064 [Photorhabdus thracensis]
MSMVKYKRSELPPLTEKRESELKALSGKSDSDIDYSDIALLNEDFWKKAAQGQFYRPVKTQASVRIDADVLVWLKKPGRG
YQTRLNAILREAMMRDLHRK

Download         Length: 303 bp

>AT53351 NZ_CP011104:c280366-280064 [Photorhabdus thracensis]
ATGAGCATGGTTAAATACAAACGAAGCGAATTGCCGCCTCTGACTGAAAAACGGGAATCTGAACTTAAAGCGCTGTCCGG
CAAGTCTGATAGTGATATTGATTACAGTGACATCGCGTTGCTGAATGAAGATTTCTGGAAGAAAGCCGCACAGGGGCAGT
TCTATCGTCCGGTAAAAACACAAGCATCAGTCAGGATTGATGCTGATGTATTGGTTTGGCTTAAAAAACCAGGAAGAGGT
TATCAGACGAGGCTTAATGCTATCTTACGTGAAGCCATGATGCGTGATTTGCATCGAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F7LJ90

Antitoxin

Source ID Structure
AlphaFold DB T0NTR3

References