Detailed information of TA system TA053348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 1040128..1040759
• Replicon: chromosome
• Accession: NZ_CP011102
• Organism: Listeria weihenstephanensis strain WS 4560

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1S7FSR4
• Locus tag: UE46_RS04960
• Protein ID: WP_036058515.1
• Coordinates: 1040415..1040759 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: UE46_RS04955
• Protein ID: WP_036058513.1
• Coordinates: 1040128..1040406 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UE46_RS04935	1035552..1037027	+	1476	WP_036058506.1	PH domain-containing protein	-
UE46_RS04940	1037056..1038444	+	1389	WP_036058507.1	protoporphyrinogen oxidase	-
UE46_RS04945	1038441..1038824	+	384	WP_036058509.1	holo-ACP synthase	-
UE46_RS04950	1038815..1039912	+	1098	WP_036058511.1	alanine racemase	-
UE46_RS04955	1040128..1040406	+	279	WP_036058513.1	CopG family transcriptional regulator	Antitoxin
UE46_RS04960	1040415..1040759	+	345	WP_036058515.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
UE46_RS04965	1041074..1041913	+	840	WP_118907809.1	STAS domain-containing protein	-
UE46_RS04970	1041918..1042274	+	357	WP_036058516.1	STAS domain-containing protein	-
UE46_RS04975	1042277..1042687	+	411	WP_036058517.1	anti-sigma regulatory factor	-
UE46_RS04980	1042700..1043713	+	1014	WP_036058519.1	PP2C family protein-serine/threonine phosphatase	-
UE46_RS04985	1043769..1044113	+	345	WP_036058520.1	STAS domain-containing protein	-
UE46_RS04990	1044097..1044570	+	474	WP_036058521.1	anti-sigma B factor RsbW	-
UE46_RS04995	1044548..1045354	+	807	WP_036058522.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12639.62 Da
• Isoelectric Point: 8.5681

>T53348 WP_036058515.1 NZ_CP011102:1040415-1040759 [Listeria weihenstephanensis]
MVKRGDVYYADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAKISKAKLPTHVEAARKHGFDRDSVILLEQVRT
IDKQRLTDKITHLDDNLMNKVNQALEISMGLVDF

Nucleotide

• Length: 345 bp

>T53348 NZ_CP011102:1040415-1040759 [Listeria weihenstephanensis]
ATGGTGAAGCGTGGGGATGTTTACTACGCGGACTTGTCTCCTGTTGTTGGAAGTGAACAAGGGGGAATACGGCCTGTTCT
CATCATTCAAAATGATATTGGCAATCGATTTAGTCCTACTGTCATCGTGGCGGCAATTACGGCAAAGATTTCTAAAGCCA
AATTGCCGACTCACGTTGAGGCAGCTCGGAAACATGGTTTTGATCGCGATTCTGTTATATTACTTGAGCAGGTTCGAACG
ATTGATAAACAACGCTTGACGGATAAGATTACGCATTTAGATGATAATCTTATGAACAAAGTAAATCAGGCGTTAGAAAT
CAGTATGGGCTTAGTAGATTTTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10625.89 Da
• Isoelectric Point: 4.2525

>AT53348 WP_036058513.1 NZ_CP011102:1040128-1040406 [Listeria weihenstephanensis]
VLEKTDTTEIWVEMTQQVLDDLDEARAKDKMGRSEMIMEATQQFLRQRKARDLHDEMERGYTEMASINFSIACECTHVES
EAEDKNIQVLGG

Nucleotide

• Length: 279 bp

>AT53348 NZ_CP011102:1040128-1040406 [Listeria weihenstephanensis]
GTGTTAGAAAAGACAGACACGACAGAAATCTGGGTAGAAATGACGCAGCAAGTATTAGACGACTTAGATGAGGCTCGGGC
AAAGGATAAAATGGGGCGGAGTGAAATGATTATGGAAGCAACGCAGCAATTTTTACGTCAAAGAAAAGCACGCGATTTGC
ATGACGAAATGGAACGTGGGTACACAGAAATGGCTTCGATTAATTTTTCGATTGCGTGTGAATGTACGCATGTCGAATCG
GAAGCTGAGGATAAAAATATACAAGTTTTAGGAGGGTGA

Structures

Toxin

• AlphaFold DB: A0A1S7FSR4