Detailed information of TA system TA053347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 441520..442082
• Replicon: chromosome
• Accession: NZ_CP011102
• Organism: Listeria weihenstephanensis strain WS 4560

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W7CSP1
• Locus tag: UE46_RS02075
• Protein ID: WP_036060452.1
• Coordinates: 441756..442082 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: UE46_RS02070
• Protein ID: WP_036060455.1
• Coordinates: 441520..441762 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UE46_RS02055	437206..438756	+	1551	WP_118907358.1	right-handed parallel beta-helix repeat-containing protein	-
UE46_RS02060	438829..439332	+	504	WP_036060458.1	hypothetical protein	-
UE46_RS02065	439379..440911	-	1533	WP_051492883.1	LPXTG cell wall anchor domain-containing protein	-
UE46_RS02070	441520..441762	+	243	WP_036060455.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
UE46_RS02075	441756..442082	+	327	WP_036060452.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
UE46_RS02080	442335..444476	+	2142	WP_051492882.1	right-handed parallel beta-helix repeat-containing protein	-
UE46_RS02085	444816..445670	+	855	WP_077912497.1	Cof-type HAD-IIB family hydrolase	-
UE46_RS02090	445720..445977	+	258	WP_036060450.1	hypothetical protein	-
UE46_RS02095	446064..446897	+	834	WP_036060447.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-100)

Antitoxin

• Antitoxin PemI-like (3-80)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12279.19 Da
• Isoelectric Point: 9.7364

>T53347 WP_036060452.1 NZ_CP011102:441756-442082 [Listeria weihenstephanensis]
MVRQGDIIKINLNPKQGYRHYICLSHRLVSDYANIAVFAPISNTSRSYPLYVSLTGTETTGKVLLDQLVTIDYNARKYNY
VETVPASLLTKLLETVKVIFQKNEKPTN

Nucleotide

• Length: 327 bp

>T53347 NZ_CP011102:441756-442082 [Listeria weihenstephanensis]
ATGGTAAGACAAGGTGATATCATCAAGATTAACCTAAATCCGAAACAAGGTTATCGCCACTATATTTGCCTCAGCCATCG
ATTAGTAAGTGACTACGCCAATATTGCTGTCTTTGCTCCTATAAGTAACACGTCGAGATCCTATCCTTTGTATGTTTCCC
TAACTGGTACAGAAACTACAGGGAAGGTATTGCTAGATCAACTGGTCACAATAGATTATAATGCTCGCAAATATAATTAT
GTAGAAACCGTACCAGCTTCTTTGCTCACGAAACTCTTAGAAACGGTAAAAGTTATTTTTCAAAAGAATGAAAAGCCAAC
TAACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9223.50 Da
• Isoelectric Point: 4.5489

>AT53347 WP_036060455.1 NZ_CP011102:441520-441762 [Listeria weihenstephanensis]
MTTVSIKKWGNSKAVRIPNNILNALNLHENDKLNIEVVDNQMILTKTVAELTIEDLFANYEGETFQTKLQEFEPMGNEKW

Nucleotide

• Length: 243 bp

>AT53347 NZ_CP011102:441520-441762 [Listeria weihenstephanensis]
ATGACCACCGTCAGTATTAAAAAGTGGGGGAATAGCAAGGCTGTTCGTATTCCCAATAATATTTTAAATGCTTTAAATCT
ACATGAGAATGACAAGCTTAATATTGAAGTTGTTGATAATCAAATGATTCTTACCAAAACAGTTGCTGAGTTGACTATTG
AGGATTTATTCGCAAATTACGAGGGTGAGACTTTCCAAACAAAGCTACAAGAATTTGAACCAATGGGGAATGAAAAATGG
TAA

Structures

Toxin

• AlphaFold DB: A0A1S7FRF6