Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 1910293..1910815 Replicon chromosome
Accession NZ_CP011099
Organism Mannheimia haemolytica strain 89010807N isolate laboratory frozen stock

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag VK67_RS09725 Protein ID WP_006249269.1
Coordinates 1910519..1910815 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A249A190
Locus tag VK67_RS09720 Protein ID WP_006249270.1
Coordinates 1910293..1910535 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VK67_RS09690 1906131..1907220 - 1090 Protein_1892 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
VK67_RS09700 1907394..1908035 - 642 WP_006249274.1 orotate phosphoribosyltransferase -
VK67_RS09705 1908158..1909000 - 843 WP_006249273.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
VK67_RS09710 1909060..1909635 + 576 WP_006249272.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
VK67_RS09715 1909613..1910281 - 669 WP_006249271.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
VK67_RS09720 1910293..1910535 - 243 WP_006249270.1 CopG family transcriptional regulator Antitoxin
VK67_RS09725 1910519..1910815 - 297 WP_006249269.1 BrnT family toxin Toxin
VK67_RS09730 1910942..1913854 - 2913 WP_006249267.1 RNA polymerase-associated protein RapA -
VK67_RS09735 1914015..1914314 - 300 WP_006249265.1 hypothetical protein -
VK67_RS09740 1914435..1915760 - 1326 WP_006249264.1 deoxyguanosinetriphosphate triphosphohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T53339 WP_006249269.1 NZ_CP011099:c1910815-1910519 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T53339 NZ_CP011099:c1910815-1910519 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9346.79 Da        Isoelectric Point: 7.1665

>AT53339 WP_006249270.1 NZ_CP011099:c1910535-1910293 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT53339 NZ_CP011099:c1910535-1910293 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGATTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A249A190

References