Detailed information of TA system TA053338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1104494..1105134
• Replicon: chromosome
• Accession: NZ_CP011099
• Organism: Mannheimia haemolytica strain 89010807N isolate laboratory frozen stock

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: VK67_RS05610
• Protein ID: WP_006250075.1
• Coordinates: 1104952..1105134 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: VK67_RS05605
• Protein ID: WP_006250074.1
• Coordinates: 1104494..1104913 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VK67_RS05585	1100007..1100813	+	807	WP_006248821.1	tryptophan synthase subunit alpha	-
VK67_RS05590	1100863..1102269	+	1407	WP_006253513.1	YdgA family protein	-
VK67_RS05595	1102336..1103376	-	1041	WP_015586960.1	IS481 family transposase	-
VK67_RS05600	1103514..1104158	-	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
VK67_RS05605	1104494..1104913	-	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
VK67_RS05610	1104952..1105134	-	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
VK67_RS05615	1105266..1105751	-	486	WP_015484250.1	hypothetical protein	-
VK67_RS05620	1105756..1106136	-	381	WP_006248823.1	hypothetical protein	-
VK67_RS05625	1106211..1106894	-	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
VK67_RS05630	1107025..1107225	+	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
VK67_RS14525	1107246..1108313	+	1068	WP_006253284.1	hypothetical protein	-
VK67_RS14420	1108323..1108481	+	159	WP_006247809.1	hypothetical protein	-
VK67_RS05640	1108490..1108789	-	300	WP_006247810.1	XRE family transcriptional regulator	-
VK67_RS05645	1108761..1109150	-	390	WP_015484247.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VK67_RS05650	1109294..1109587	+	294	WP_006247812.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1046547..1169007	122460
	-	flank	IS/Tn	-	-	1102336..1103376	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T53338 WP_006250075.1 NZ_CP011099:c1105134-1104952 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T53338 NZ_CP011099:c1105134-1104952 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT53338 WP_006250074.1 NZ_CP011099:c1104913-1104494 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT53338 NZ_CP011099:c1104913-1104494 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9