Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 885845..886384 Replicon chromosome
Accession NZ_CP011099
Organism Mannheimia haemolytica strain 89010807N isolate laboratory frozen stock

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag VK67_RS04470 Protein ID WP_006247804.1
Coordinates 885845..886114 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag VK67_RS04475 Protein ID WP_006247803.1
Coordinates 886118..886384 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VK67_RS04425 880979..881278 + 300 WP_006247810.1 XRE family transcriptional regulator -
VK67_RS14415 881287..881445 - 159 WP_006247809.1 hypothetical protein -
VK67_RS04430 881455..882141 - 687 WP_006247808.1 hypothetical protein -
VK67_RS04435 882252..882863 - 612 WP_006253310.1 hypothetical protein -
VK67_RS04440 882912..883538 - 627 WP_006247807.1 tail assembly protein -
VK67_RS04450 883760..884479 - 720 WP_006247806.1 preprotein translocase subunit YajC -
VK67_RS04455 884469..884759 - 291 Protein_839 C40 family peptidase -
VK67_RS04460 884737..885447 - 711 WP_006253312.1 tape measure protein -
VK67_RS04465 885507..885770 - 264 WP_006247805.1 hypothetical protein -
VK67_RS04470 885845..886114 - 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
VK67_RS04475 886118..886384 - 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
VK67_RS04480 886452..886682 - 231 WP_006247802.1 DUF4035 domain-containing protein -
VK67_RS04485 886727..887128 - 402 WP_006247801.1 hypothetical protein -
VK67_RS04490 887211..887852 - 642 WP_006247800.1 hypothetical protein -
VK67_RS04495 887884..888279 - 396 WP_006247799.1 phage tail protein -
VK67_RS13870 888304..888531 - 228 Protein_848 phage terminase large subunit family protein -
VK67_RS04500 888681..889055 + 375 WP_006247798.1 hypothetical protein -
VK67_RS04505 889063..889230 + 168 WP_006253314.1 DNA cytosine methyltransferase -
VK67_RS13875 889311..889634 + 324 WP_006253316.1 helix-turn-helix domain-containing protein -
VK67_RS04510 889538..890248 + 711 Protein_852 tyrosine-type recombinase/integrase -
VK67_RS04515 890349..890984 + 636 Protein_853 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 878504..898997 20493
- flank IS/Tn - - 890349..891041 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T53337 WP_006247804.1 NZ_CP011099:c886114-885845 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T53337 NZ_CP011099:c886114-885845 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT53337 WP_006247803.1 NZ_CP011099:c886384-886118 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT53337 NZ_CP011099:c886384-886118 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References