Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1109054..1109694 Replicon chromosome
Accession NZ_CP011098
Organism Mannheimia haemolytica strain 89010807N isolate laboratory frozen stock

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag WC39_RS05665 Protein ID WP_006250075.1
Coordinates 1109054..1109236 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NFW9
Locus tag WC39_RS05670 Protein ID WP_006250074.1
Coordinates 1109275..1109694 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WC39_RS05625 1104601..1104894 - 294 WP_006247812.1 hypothetical protein -
WC39_RS05630 1105038..1105427 + 390 WP_015484247.1 type II toxin-antitoxin system RelE/ParE family toxin -
WC39_RS05635 1105399..1105698 + 300 WP_006247810.1 XRE family transcriptional regulator -
WC39_RS14430 1105707..1105865 - 159 WP_006247809.1 hypothetical protein -
WC39_RS14550 1105875..1106942 - 1068 WP_006253284.1 hypothetical protein -
WC39_RS05645 1106963..1107163 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
WC39_RS05650 1107294..1107977 + 684 WP_006248824.1 helix-turn-helix transcriptional regulator -
WC39_RS05655 1108052..1108432 + 381 WP_006248823.1 hypothetical protein -
WC39_RS05660 1108437..1108922 + 486 WP_015484250.1 hypothetical protein -
WC39_RS05665 1109054..1109236 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
WC39_RS05670 1109275..1109694 + 420 WP_006250074.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
WC39_RS05675 1110030..1110674 + 645 WP_006253274.1 tyrosine-type recombinase/integrase -
WC39_RS05680 1110812..1111852 + 1041 WP_015586960.1 IS481 family transposase -
WC39_RS05685 1111919..1113325 - 1407 WP_006253513.1 YdgA family protein -
WC39_RS05690 1113375..1114181 - 807 WP_006248821.1 tryptophan synthase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1110812..1111852 1040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T53330 WP_006250075.1 NZ_CP011098:1109054-1109236 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T53330 NZ_CP011098:1109054-1109236 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15938.30 Da        Isoelectric Point: 4.6963

>AT53330 WP_006250074.1 NZ_CP011098:1109275-1109694 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Download         Length: 420 bp

>AT53330 NZ_CP011098:1109275-1109694 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NFW9

References