Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 885827..886366 Replicon chromosome
Accession NZ_CP011098
Organism Mannheimia haemolytica strain 89010807N isolate laboratory frozen stock

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag WC39_RS04470 Protein ID WP_006247804.1
Coordinates 885827..886096 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag WC39_RS04475 Protein ID WP_006247803.1
Coordinates 886100..886366 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WC39_RS04425 880961..881260 + 300 WP_006247810.1 XRE family transcriptional regulator -
WC39_RS14415 881269..881427 - 159 WP_006247809.1 hypothetical protein -
WC39_RS04430 881437..882123 - 687 WP_006247808.1 hypothetical protein -
WC39_RS04435 882234..882845 - 612 WP_006253310.1 hypothetical protein -
WC39_RS04440 882894..883520 - 627 WP_006247807.1 tail assembly protein -
WC39_RS04450 883742..884461 - 720 WP_006247806.1 preprotein translocase subunit YajC -
WC39_RS04455 884451..884741 - 291 Protein_839 C40 family peptidase -
WC39_RS04460 884719..885429 - 711 WP_006253312.1 tape measure protein -
WC39_RS04465 885489..885752 - 264 WP_006247805.1 hypothetical protein -
WC39_RS04470 885827..886096 - 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
WC39_RS04475 886100..886366 - 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
WC39_RS04480 886434..886664 - 231 WP_006247802.1 DUF4035 domain-containing protein -
WC39_RS04485 886709..887110 - 402 WP_006247801.1 hypothetical protein -
WC39_RS04490 887193..887834 - 642 WP_006247800.1 hypothetical protein -
WC39_RS04495 887866..888261 - 396 WP_006247799.1 phage tail protein -
WC39_RS13855 888286..888513 - 228 Protein_848 phage terminase large subunit family protein -
WC39_RS04500 888663..889037 + 375 WP_006247798.1 hypothetical protein -
WC39_RS04505 889045..889212 + 168 WP_006253314.1 DNA cytosine methyltransferase -
WC39_RS13860 889293..889616 + 324 WP_006253316.1 helix-turn-helix domain-containing protein -
WC39_RS04510 889520..890230 + 711 Protein_852 tyrosine-type recombinase/integrase -
WC39_RS04515 890331..890966 + 636 Protein_853 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 874773..898979 24206
- flank IS/Tn - - 890331..891023 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T53329 WP_006247804.1 NZ_CP011098:c886096-885827 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T53329 NZ_CP011098:c886096-885827 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT53329 WP_006247803.1 NZ_CP011098:c886366-886100 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT53329 NZ_CP011098:c886366-886100 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References