Detailed information of TA system TA053325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 80601..81316
• Replicon: chromosome
• Accession: NZ_CP011098
• Organism: Mannheimia haemolytica strain 89010807N isolate laboratory frozen stock

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WC39_RS00390
• Protein ID: WP_006250491.1
• Coordinates: 80601..81029 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A248ZW87
• Locus tag: WC39_RS00395
• Protein ID: WP_006248272.1
• Coordinates: 81029..81316 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WC39_RS00375	75855..77507	+	1653	WP_006248275.1	phospho-sugar mutase	-
WC39_RS00380	77560..78494	+	935	Protein_78	nucleoside hydrolase	-
WC39_RS00385	78516..80597	-	2082	WP_006248274.1	ATP-dependent DNA helicase RecG	-
WC39_RS00390	80601..81029	-	429	WP_006250491.1	type II toxin-antitoxin system VapC family toxin	Toxin
WC39_RS00395	81029..81316	-	288	WP_006248272.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
WC39_RS00400	81480..83363	+	1884	WP_006248270.1	molecular chaperone HtpG	-
WC39_RS14475	83416..83577	+	162	WP_015483997.1	hypothetical protein	-
WC39_RS00405	83741..85456	+	1716	WP_006248268.1	proline--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16640.06 Da
• Isoelectric Point: 8.9269

>T53325 WP_006250491.1 NZ_CP011098:c81029-80601 [Mannheimia haemolytica]
MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCITLSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQT
KAFVVNNDIALLASEYHIPNKMDLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA

Nucleotide

• Length: 429 bp

>T53325 NZ_CP011098:c81029-80601 [Mannheimia haemolytica]
ATGTATCGCTACCTTTTTGATACCAATATAGTTTCCGAGCTTTATAAGTTAAGAAATGACAAGATGGATGTCAATGTTCG
CCAGTGGCTGAGAGGGGTTAATCCTAGCCAGACCAATATCAGCTGTATTACGTTATCTGAAATCAAAACAGGTATTTTGC
TGAAAGCCCGCAAAGACAAAGAACAATCAGACCGATTAAATGATTGGTTTGAAAATAATGTATTAAAGGCTTATCAAACA
AAAGCGTTTGTGGTGAATAACGACATTGCTCTATTGGCATCAGAGTACCATATTCCCAATAAAATGGATTTAAATGATGC
TTATATTGCGGCAACCGCCAAATACCACAATCTGATTTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGC
GTTTATTTAACCCATTTGAAACCGCATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11085.39 Da
• Isoelectric Point: 4.3648

>AT53325 WP_006248272.1 NZ_CP011098:c81316-81029 [Mannheimia haemolytica]
MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDYQQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVE
IRKSNKPRPVDFGDE

Nucleotide

• Length: 288 bp

>AT53325 NZ_CP011098:c81316-81029 [Mannheimia haemolytica]
ATGGCAATAACCATTACTTCCAGAGAATTTAATCAAAATGCCAGCAAAGTGTTAAAAATGGCAGAAAAAGAGCCGGTTTT
TATTACCAAATGGGGCAAAATCGTCAATGTGGTGTCTAGTTATATGGATTATCAGCAGAAAAAGCCTAGCGAGCCAAGCT
TTGAAGAGCTATTCGCCTCTTCCGAAAATGAGGATGTGAGCGATGAATTTTGGCAAGAGTTTGAACAAACATTGGTTGAA
ATCCGCAAAAGCAATAAACCAAGACCTGTTGATTTTGGAGATGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A248ZW87