Detailed information of TA system TA053323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 2757361..2758699
• Replicon: chromosome
• Accession: NZ_CP011078
• Organism: Yersinia ruckeri strain Big Creek 74

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: UGYR_RS12505
• Protein ID: WP_045844434.1
• Coordinates: 2757749..2758699 (+)
• Length: 317 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A848MNT5
• Locus tag: UGYR_RS12500
• Protein ID: WP_038245317.1
• Coordinates: 2757361..2757756 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UGYR_RS12470	2752817..2753239	+	423	WP_052690340.1	restriction endonuclease subunit S	-
UGYR_RS12475	2753333..2753908	+	576	WP_045844432.1	DUF4433 domain-containing protein	-
UGYR_RS12480	2753911..2754567	+	657	WP_045844433.1	DUF4433 domain-containing protein	-
UGYR_RS12485	2754564..2755631	+	1068	WP_038245756.1	macro domain-containing protein	-
UGYR_RS17065	2755652..2755918	+	267	Protein_2414	hypothetical protein	-
UGYR_RS17070	2755937..2756335	-	399	Protein_2415	DDE-type integrase/transposase/recombinase	-
UGYR_RS12490	2756340..2756630	+	291	Protein_2416	transposase	-
UGYR_RS17510	2756644..2756742	-	99	Protein_2417	transposase	-
UGYR_RS17515	2756778..2756906	-	129	Protein_2418	helix-turn-helix domain-containing protein	-
UGYR_RS12495	2757056..2757286	+	231	WP_073990333.1	DUF4942 domain-containing protein	-
UGYR_RS12500	2757361..2757756	+	396	WP_038245317.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
UGYR_RS12505	2757749..2758699	+	951	WP_045844434.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
UGYR_RS17325	2759072..2759356	+	285	WP_038245319.1	antirestriction protein	-
UGYR_RS12515	2760020..2761174	+	1155	WP_038245380.1	N-acetylneuraminate epimerase	-
UGYR_RS12520	2761222..2761914	+	693	WP_038245321.1	membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2756382..2756630	248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (37-304)

Antitoxin

• Protein of unknown function DUF6088 (10-114)

Sequences

Toxin

Protein

• Length: 317 a.a.
• Molecular weight: 35995.30 Da
• Isoelectric Point: 4.5092

>T53323 WP_045844434.1 NZ_CP011078:2757749-2758699 [Yersinia ruckeri]
MDDLTKLFPDVADALGIESVSIVEKDYYIVELLRLLQPLDFDTHQLVFAGGTALSKAGISLNRMSEDVDIKLVPLAGFSQ
QYSRGQRKSIRKGIIQTIIETVAASGTFLLDEEYPKVTRDEYRYNEIPVRYPQEYVQVPCLRPFIKLELMETDLLEAAEN
RAISSLVMDLTKKGEVVRAFPCATIGSTQAEKLISMMRRTAASMRDVERAVDESLVRHIYDNFCIVREKGADVPVLKQFV
QECIEQDIERYGAQYPEFCESPVEELKMGLEELVSNPVYKERYLQFVTPMVFGESHVSWEEAYACFRKTVLDVVDA

Nucleotide

• Length: 951 bp

>T53323 NZ_CP011078:2757749-2758699 [Yersinia ruckeri]
ATGGATGATTTAACAAAGCTATTCCCAGATGTTGCTGATGCTCTGGGCATTGAATCTGTTTCAATTGTAGAAAAAGATTA
TTACATCGTTGAGTTATTACGATTGCTCCAGCCTCTGGACTTTGACACGCATCAGCTGGTTTTTGCTGGTGGAACAGCAT
TGTCAAAAGCGGGTATATCTTTGAACAGGATGTCGGAGGACGTGGACATTAAGCTTGTACCTCTCGCCGGTTTTTCCCAG
CAATATTCCAGAGGTCAGCGTAAAAGCATCCGTAAGGGTATTATTCAGACCATTATCGAGACGGTTGCCGCCTCAGGTAC
GTTTCTCCTGGATGAAGAGTATCCAAAGGTTACTCGTGATGAATACCGTTATAATGAAATTCCGGTGCGTTACCCGCAGG
AATATGTACAAGTTCCCTGCTTAAGGCCATTTATCAAACTGGAACTGATGGAAACGGATTTGCTTGAGGCTGCGGAAAAT
CGTGCTATTAGCTCCTTGGTAATGGATCTAACAAAGAAGGGCGAAGTAGTGAGGGCTTTCCCTTGCGCAACCATCGGTAG
CACGCAGGCTGAAAAACTGATCTCGATGATGCGCAGAACGGCTGCAAGTATGCGTGATGTGGAACGTGCAGTGGATGAAT
CACTGGTACGTCATATCTACGATAATTTTTGTATTGTTAGAGAAAAAGGTGCTGACGTTCCTGTTTTGAAGCAATTCGTG
CAGGAATGCATTGAACAGGATATTGAACGTTACGGTGCTCAGTACCCTGAATTCTGCGAGTCGCCTGTCGAAGAACTAAA
AATGGGGCTGGAAGAACTCGTGAGCAATCCTGTTTATAAGGAACGCTATCTGCAATTCGTCACACCAATGGTCTTCGGTG
AAAGCCACGTTTCATGGGAAGAGGCCTATGCCTGTTTTCGCAAAACGGTATTGGATGTGGTTGATGCATAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14406.76 Da
• Isoelectric Point: 11.0360

>AT53323 WP_038245317.1 NZ_CP011078:2757361-2757756 [Yersinia ruckeri]
MTTKARIQLRLKRSKRYVFTRDDFKDLAGYDQIGRALRELVKEGQLLKVGYGVYTKARQNVITGKLMPAAPGGSAAVVIE
ALERLKVDYRLVGATSAYNSGKSTQIPASLEIKTSPRFKRTLSVGSSKLNG

Nucleotide

• Length: 396 bp

>AT53323 NZ_CP011078:2757361-2757756 [Yersinia ruckeri]
ATGACTACAAAAGCTCGTATTCAGTTACGACTGAAACGCTCAAAGCGTTATGTTTTCACTCGTGACGATTTCAAAGATCT
TGCTGGCTACGACCAAATTGGTAGAGCACTGCGTGAACTGGTGAAGGAAGGGCAGCTGCTGAAAGTAGGCTATGGCGTCT
ACACCAAAGCGCGGCAAAATGTGATTACCGGTAAGTTGATGCCTGCGGCTCCCGGAGGTTCGGCTGCGGTAGTGATTGAG
GCGTTGGAACGTCTGAAAGTGGATTACCGCTTGGTTGGAGCAACAAGCGCCTATAATAGCGGTAAATCCACTCAGATCCC
CGCTTCACTGGAAATCAAAACCTCACCACGTTTTAAGAGGACATTGTCAGTAGGGAGCAGCAAACTAAATGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A848MNT5