Detailed information of TA system TA053321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2752019..2752553
• Replicon: chromosome
• Accession: NZ_CP011078
• Organism: Yersinia ruckeri strain Big Creek 74

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0T9LYG3
• Locus tag: UGYR_RS12465
• Protein ID: WP_038275268.1
• Coordinates: 2752263..2752553 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A1JJ37
• Locus tag: UGYR_RS12460
• Protein ID: WP_005175264.1
• Coordinates: 2752019..2752273 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UGYR_RS12445	2747635..2748722	+	1088	WP_099511755.1	IS3 family transposase	-
UGYR_RS12450	2749127..2750071	-	945	WP_038275265.1	hypothetical protein	-
UGYR_RS12455	2750586..2751845	+	1260	Protein_2407	integrase arm-type DNA-binding domain-containing protein	-
UGYR_RS12460	2752019..2752273	+	255	WP_005175264.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
UGYR_RS12465	2752263..2752553	+	291	WP_038275268.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
UGYR_RS12470	2752817..2753239	+	423	WP_052690340.1	restriction endonuclease subunit S	-
UGYR_RS12475	2753333..2753908	+	576	WP_045844432.1	DUF4433 domain-containing protein	-
UGYR_RS12480	2753911..2754567	+	657	WP_045844433.1	DUF4433 domain-containing protein	-
UGYR_RS12485	2754564..2755631	+	1068	WP_038245756.1	macro domain-containing protein	-
UGYR_RS17065	2755652..2755918	+	267	Protein_2414	hypothetical protein	-
UGYR_RS17070	2755937..2756335	-	399	Protein_2415	DDE-type integrase/transposase/recombinase	-
UGYR_RS12490	2756340..2756630	+	291	Protein_2416	transposase	-
UGYR_RS17510	2756644..2756742	-	99	Protein_2417	transposase	-
UGYR_RS17515	2756778..2756906	-	129	Protein_2418	helix-turn-helix domain-containing protein	-
UGYR_RS12495	2757056..2757286	+	231	WP_073990333.1	DUF4942 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2727395..2755631	28236
	-	flank	IS/Tn	-	-	2756382..2756630	248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-75)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11494.42 Da
• Isoelectric Point: 9.9816

>T53321 WP_038275268.1 NZ_CP011078:2752263-2752553 [Yersinia ruckeri]
MTFNIDFDERALKEWHKLDKTIREQFKKKLRKLQENPYIESARLHGDLAGCFKIKLRASGFRLIYQVIDEEIVIWIVAVG
KREDEKAYDIAKKRIQ

Nucleotide

• Length: 291 bp

>T53321 NZ_CP011078:2752263-2752553 [Yersinia ruckeri]
ATGACCTTTAATATCGACTTTGACGAACGGGCATTAAAAGAATGGCACAAACTAGATAAAACTATCCGAGAGCAATTTAA
AAAGAAACTAAGAAAATTACAGGAAAATCCCTATATTGAGTCAGCACGTTTGCACGGTGATCTGGCTGGTTGTTTCAAAA
TAAAATTACGCGCATCTGGCTTTCGGTTGATTTACCAAGTTATCGATGAGGAGATTGTGATTTGGATCGTTGCTGTTGGT
AAACGGGAAGATGAAAAAGCTTATGATATTGCCAAGAAGCGAATCCAATAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9680.07 Da
• Isoelectric Point: 4.8283

>AT53321 WP_005175264.1 NZ_CP011078:2752019-2752273 [Yersinia ruckeri]
MSTIQIRVDDELKKSAYQAFEKLNLSPSDALRLFLRYVAENEKLPFSEVSVMVSNQDEDADILAVVRERLKNPAKRIRVN
IDDL

Nucleotide

• Length: 255 bp

>AT53321 NZ_CP011078:2752019-2752273 [Yersinia ruckeri]
ATGAGTACTATCCAAATTCGTGTGGATGATGAACTGAAGAAAAGTGCATATCAGGCGTTTGAAAAACTGAATCTTTCGCC
TTCTGATGCACTACGCCTGTTCCTACGTTATGTTGCAGAGAACGAAAAGCTGCCTTTTTCAGAAGTATCCGTCATGGTCA
GTAATCAAGATGAGGATGCTGATATTTTGGCTGTTGTCCGTGAACGTTTGAAAAATCCTGCAAAGCGTATCAGAGTAAAT
ATTGATGACCTTTAA

Structures

Toxin

• AlphaFold DB: A0A0T9LYG3

Antitoxin

• AlphaFold DB: A1JJ37