Detailed information of TA system TA053320

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yfjZ/CbtA-CbeA
• Location: 2733970..2734706
• Replicon: chromosome
• Accession: NZ_CP011078
• Organism: Yersinia ruckeri strain Big Creek 74

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: UGYR_RS12360
• Protein ID: WP_045844421.1
• Coordinates: 2733970..2734341 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: UGYR_RS12365
• Protein ID: WP_045844422.1
• Coordinates: 2734338..2734706 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UGYR_RS12335	2729798..2730235	+	438	WP_045844416.1	hypothetical protein	-
UGYR_RS12340	2730255..2731523	+	1269	WP_045844417.1	hypothetical protein	-
UGYR_RS12345	2731746..2732183	+	438	WP_045844418.1	hypothetical protein	-
UGYR_RS12350	2732266..2733087	+	822	WP_045844419.1	DUF945 domain-containing protein	-
UGYR_RS12355	2733447..2733878	+	432	WP_045844420.1	antirestriction protein	-
UGYR_RS12360	2733970..2734341	+	372	WP_045844421.1	TA system toxin CbtA family protein	Toxin
UGYR_RS12365	2734338..2734706	+	369	WP_045844422.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
UGYR_RS12370	2735172..2736422	+	1251	Protein_2396	integrase arm-type DNA-binding domain-containing protein	-
UGYR_RS12375	2736534..2737340	+	807	Protein_2397	hypothetical protein	-
UGYR_RS12380	2737511..2737717	+	207	WP_045844423.1	AlpA family transcriptional regulator	-
UGYR_RS12390	2737892..2739042	+	1151	WP_155393436.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2727395..2755631	28236

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (12-101)

Antitoxin

• Antitoxin CbeA (10-108)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13580.72 Da
• Isoelectric Point: 6.6362

>T53320 WP_045844421.1 NZ_CP011078:2733970-2734341 [Yersinia ruckeri]
MKPCPITPGALPVPVIYQQLLAFLLEHHYGLSLNDTPYHDEQAIAQQIEQGISVGDTINTLVEKYALVRIGRNGFSVLAQ
DPMLSKGDIVRARQALGLWRAHFPPTLLHPTEPLNPIPQSPLT

Nucleotide

• Length: 372 bp

>T53320 NZ_CP011078:2733970-2734341 [Yersinia ruckeri]
ATGAAACCATGTCCCATCACACCGGGCGCATTACCCGTGCCTGTTATTTATCAACAACTGCTCGCTTTCCTGCTGGAGCA
CCACTACGGCCTGAGCCTCAACGATACGCCCTATCACGATGAACAGGCGATTGCGCAACAGATAGAACAAGGGATTTCAG
TCGGTGACACCATCAACACACTGGTTGAGAAATATGCATTAGTGCGAATTGGTCGTAACGGGTTCAGCGTACTGGCACAA
GACCCGATGTTGAGTAAGGGCGATATCGTGCGGGCTCGCCAGGCTTTGGGCTTGTGGCGTGCTCATTTTCCGCCCACCTT
GCTGCACCCAACCGAACCCCTCAATCCTATTCCACAGAGTCCGTTAACATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13224.95 Da
• Isoelectric Point: 5.8684

>AT53320 WP_045844422.1 NZ_CP011078:2734338-2734706 [Yersinia ruckeri]
MNNHMTTSPTWGLKRDITPCLGARLVQEGTHLHFLSDRANIVGTFSPQTTQSLDVAFPALITKLESQLLSGALDPHRQQG
ITVRHGEFVCDGDTLGSHGYVYIAVYQELPLLLSEGGGPPDV

Nucleotide

• Length: 369 bp

>AT53320 NZ_CP011078:2734338-2734706 [Yersinia ruckeri]
ATGAATAACCACATGACTACTTCCCCAACATGGGGCCTGAAACGTGACATTACGCCATGTTTGGGAGCCAGGCTGGTACA
GGAGGGAACACACCTGCACTTTCTGAGTGACAGAGCCAATATTGTCGGCACCTTTTCCCCTCAAACCACTCAGTCTCTTG
ATGTGGCATTCCCCGCGCTGATAACCAAACTGGAGTCGCAGCTGCTGTCCGGGGCGCTGGATCCACACCGTCAGCAAGGC
ATTACGGTTCGGCATGGCGAATTTGTCTGTGACGGCGATACGCTGGGCAGTCACGGTTATGTCTATATCGCGGTGTATCA
GGAACTGCCTTTGTTACTTTCAGAAGGCGGTGGGCCACCTGACGTTTAA