Detailed information of TA system TA053308

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4300089..4300758
• Replicon: chromosome
• Accession: NZ_CP011077
• Organism: Klebsiella michiganensis strain RC10

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: VW41_RS19870
• Protein ID: WP_045783894.1
• Coordinates: 4300089..4300511 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A1X7KKT1
• Locus tag: VW41_RS19875
• Protein ID: WP_008461626.1
• Coordinates: 4300492..4300758 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VW41_RS19850	4296089..4297822	-	1734	WP_045783890.1	single-stranded-DNA-specific exonuclease RecJ	-
VW41_RS19855	4297829..4298545	-	717	WP_045783891.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
VW41_RS19860	4298572..4299468	-	897	WP_045783892.1	site-specific tyrosine recombinase XerD	-
VW41_RS19865	4299571..4300092	+	522	WP_045783893.1	flavodoxin FldB	-
VW41_RS19870	4300089..4300511	-	423	WP_045783894.1	protein YgfX	Toxin
VW41_RS19875	4300492..4300758	-	267	WP_008461626.1	FAD assembly factor SdhE	Antitoxin
VW41_RS19880	4301005..4301991	+	987	WP_045783895.1	tRNA-modifying protein YgfZ	-
VW41_RS19885	4302046..4302699	-	654	WP_045783896.1	hemolysin III family protein	-
VW41_RS19890	4302875..4303468	+	594	WP_045783897.1	HD domain-containing protein	-
VW41_RS19895	4303511..4304239	+	729	WP_045783898.1	MurR/RpiR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 16537.49 Da
• Isoelectric Point: 11.7122

>T53308 WP_045783894.1 NZ_CP011077:c4300511-4300089 [Klebsiella michiganensis]
VVLWQSDLRVSWRAQWMSLLLHGLVALFVLLMPWPMSYTLVWMLLLSLVVFDSVRSQRRIHARQGEIKLLTDFRLRWQGR
DWDIVGNPWIIDSGVMLRLRRAGKQRCQHLWLAADSMDIGEWRDLRRLLMQQQASGSGQV

Nucleotide

• Length: 423 bp

>T53308 NZ_CP011077:c4300511-4300089 [Klebsiella michiganensis]
GTGGTCCTGTGGCAATCTGACCTGCGGGTTTCATGGCGCGCGCAATGGATGTCATTACTCCTCCACGGCCTGGTGGCGCT
GTTCGTGCTGCTAATGCCGTGGCCGATGAGCTACACGCTGGTCTGGATGCTATTGCTTTCCCTTGTGGTTTTTGACAGCG
TGCGCAGCCAGCGCCGTATTCACGCTCGCCAGGGCGAAATTAAACTGCTGACGGACTTTCGTCTGCGCTGGCAAGGGCGT
GACTGGGATATCGTCGGAAACCCGTGGATTATTGATAGCGGGGTGATGCTAAGACTGCGACGGGCTGGAAAACAACGTTG
CCAGCACCTTTGGCTGGCCGCTGACAGCATGGACATCGGTGAATGGCGTGACTTACGCCGCCTGCTGATGCAGCAACAGG
CGAGCGGAAGCGGGCAGGTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10385.88 Da
• Isoelectric Point: 5.6202

>AT53308 WP_008461626.1 NZ_CP011077:c4300758-4300492 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYETLSDSDKQLFVRLLESDDPDLFNWLMNHGKPADAELQRMVALIQTRN
KERGPVAI

Nucleotide

• Length: 267 bp

>AT53308 NZ_CP011077:c4300758-4300492 [Klebsiella michiganensis]
ATGGATATCAATAATAAAGCCCGAATTCACTGGGCATGCCGCCGTGGAATGCGCGAGCTCGATATTTCGATTATGCCGTT
CTTCGAACATGAATACGAAACGCTTAGCGACAGCGACAAGCAGCTGTTTGTCCGCCTGCTGGAGTCTGACGATCCAGACT
TGTTTAACTGGTTGATGAACCACGGTAAACCAGCAGATGCCGAACTTCAGCGTATGGTTGCCCTGATTCAGACACGGAAT
AAAGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1X7KKT1