Detailed information of TA system TA053307

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4256817..4257478
• Replicon: chromosome
• Accession: NZ_CP011077
• Organism: Klebsiella michiganensis strain RC10

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: S3IMJ6
• Locus tag: VW41_RS19620
• Protein ID: WP_000698542.1
• Coordinates: 4256817..4257140 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: S3J179
• Locus tag: VW41_RS19625
• Protein ID: WP_000065326.1
• Coordinates: 4257161..4257478 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VW41_RS19605	4252126..4252854	+	729	WP_045783880.1	hypothetical protein	-
VW41_RS19610	4254140..4254472	+	333	WP_045783881.1	NIPSNAP family protein	-
VW41_RS19615	4254588..4256519	-	1932	WP_045783882.1	site-specific integrase	-
VW41_RS19620	4256817..4257140	-	324	WP_000698542.1	TA system toxin CbtA family protein	Toxin
VW41_RS19625	4257161..4257478	-	318	WP_000065326.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
VW41_RS19630	4257497..4257718	-	222	WP_000691995.1	DUF987 domain-containing protein	-
VW41_RS19635	4257727..4258203	-	477	WP_000811694.1	RadC family protein	-
VW41_RS19640	4258219..4258677	-	459	WP_016538084.1	antirestriction protein	-
VW41_RS19645	4258763..4258999	-	237	WP_000004273.1	DUF905 domain-containing protein	-
VW41_RS19650	4259077..4259487	-	411	WP_000912997.1	hypothetical protein	-
VW41_RS19655	4259554..4259991	-	438	WP_024198335.1	hypothetical protein	-
VW41_RS19660	4260033..4260569	-	537	WP_000219797.1	DUF4339 domain-containing protein	-
VW41_RS19665	4260595..4261305	-	711	WP_024198336.1	hypothetical protein	-
VW41_RS19670	4261514..4262338	-	825	WP_000197385.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4246701..4288377	41676

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12350.38 Da
• Isoelectric Point: 8.9789

>T53307 WP_000698542.1 NZ_CP011077:c4257140-4256817 [Klebsiella michiganensis]
MKILPATISRAAKPCLPPVAVWQLLLTRLLEKHYGLTLNDTPFSDETVIKEHFDAGITLANAINFLVEKYELVRIDRRGF
SWQEQTPYLTNIDIMRARRDLGLLNRN

Nucleotide

• Length: 324 bp

>T53307 NZ_CP011077:c4257140-4256817 [Klebsiella michiganensis]
ATGAAAATTCTACCGGCTACAATTTCGCGGGCGGCGAAGCCCTGCCTGCCACCCGTTGCAGTCTGGCAATTGCTTCTTAC
ACGGCTACTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCAGTGATGAAACCGTTATAAAGGAACATTTCG
ATGCCGGTATCACTCTCGCTAATGCCATTAACTTTCTAGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTT
AGCTGGCAAGAACAAACGCCATATCTCACGAATATTGATATCATGAGGGCCCGCCGCGATCTGGGTTTATTAAATCGTAA
TTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11830.50 Da
• Isoelectric Point: 6.9367

>AT53307 WP_000065326.1 NZ_CP011077:c4257478-4257161 [Klebsiella michiganensis]
MSNIIWGLQRDITPRLGTRLVQEGNQLHYLADRACITGKFSDAECLKLDVAFPHFISRMESMLSTGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYVAVYSTQR

Nucleotide

• Length: 318 bp

>AT53307 NZ_CP011077:c4257478-4257161 [Klebsiella michiganensis]
ATGAGCAACATCATTTGGGGCCTGCAGCGCGATATCACGCCGCGCCTGGGAACACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTACCTGGCTGACCGGGCCTGTATCACAGGTAAGTTCAGTGATGCCGAATGTCTTAAGCTGGATGTGGCATTTCCGC
ATTTTATCAGCCGGATGGAATCCATGCTGAGCACGGGTGAAATGAATCCTCGCCACGCCCACTGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGTGGCTATGTATACGTCGCTGTTTATTCCACTCAGCGCTAA

Structures

Toxin

• AlphaFold DB: S3IMJ6

Antitoxin

• AlphaFold DB: S3J179