Detailed information of TA system TA053284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 91681..92282
• Replicon: plasmid pSJ_98
• Accession: NZ_CP011063
• Organism: Escherichia coli str. Sanji

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: Y979_RS26055
• Protein ID: WP_001216045.1
• Coordinates: 91902..92282 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: Y979_RS26050
• Protein ID: WP_001190712.1
• Coordinates: 91681..91902 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y979_RS26005	86867..87130	+	264	WP_000224220.1	hypothetical protein	-
Y979_RS26010	87141..87740	+	600	WP_053903357.1	DUF551 domain-containing protein	-
Y979_RS26015	87823..88056	+	234	WP_000516537.1	hypothetical protein	-
Y979_RS26020	88235..88528	+	294	WP_001677496.1	hypothetical protein	-
Y979_RS26025	88535..88909	+	375	WP_023154376.1	hypothetical protein	-
Y979_RS26030	88891..89823	+	933	WP_077780118.1	hypothetical protein	-
Y979_RS26035	89820..90182	+	363	WP_001261544.1	hypothetical protein	-
Y979_RS31545	90257..90394	-	138	WP_000123562.1	hypothetical protein	-
Y979_RS26040	90844..91095	+	252	WP_001377386.1	DNA polymerase III subunit theta	-
Y979_RS26045	91219..91608	+	390	WP_000506726.1	DNA repair protein	-
Y979_RS26050	91681..91902	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
Y979_RS26055	91902..92282	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
Y979_RS26060	92287..92466	+	180	WP_000113019.1	hypothetical protein	-
Y979_RS26065	92494..93537	+	1044	WP_000648823.1	DUF968 domain-containing protein	-
Y979_RS26070	93626..94078	+	453	WP_001369802.1	hypothetical protein	-
Y979_RS26075	94165..95358	+	1194	WP_000219605.1	terminase	-
Y979_RS26080	95358..96842	+	1485	WP_000124155.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..98436	98436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T53284 WP_001216045.1 NZ_CP011063:91902-92282 [Escherichia coli str. Sanji]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T53284 NZ_CP011063:91902-92282 [Escherichia coli str. Sanji]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT53284 WP_001190712.1 NZ_CP011063:91681-91902 [Escherichia coli str. Sanji]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT53284 NZ_CP011063:91681-91902 [Escherichia coli str. Sanji]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6