Detailed information of TA system TA053283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4838296..4838872
• Replicon: chromosome
• Accession: NZ_CP011061
• Organism: Escherichia coli str. Sanji

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: Y979_RS23870
• Protein ID: WP_001297643.1
• Coordinates: 4838296..4838583 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A080I7H7
• Locus tag: Y979_RS23875
• Protein ID: WP_000063160.1
• Coordinates: 4838570..4838872 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y979_RS23845	4834533..4835813	-	1281	WP_001037390.1	DUF445 domain-containing protein	-
Y979_RS23850	4835854..4837086	-	1233	WP_001137030.1	multidrug efflux MFS transporter MdtM	-
Y979_RS30180	4837401..4838097	+	697	Protein_4533	IS1 family transposase	-
Y979_RS23870	4838296..4838583	+	288	WP_001297643.1	BrnT family toxin	Toxin
Y979_RS23875	4838570..4838872	+	303	WP_000063160.1	BrnA antitoxin family protein	Antitoxin
Y979_RS23880	4838906..4839862	-	957	WP_001297640.1	GTPase	-
Y979_RS23885	4839873..4840076	-	204	WP_000467859.1	YbdD/YjiX family protein	-
Y979_RS23890	4840126..4842276	-	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4837888..4838097	209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T53283 WP_001297643.1 NZ_CP011061:4838296-4838583 [Escherichia coli str. Sanji]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T53283 NZ_CP011061:4838296-4838583 [Escherichia coli str. Sanji]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11552.01 Da
• Isoelectric Point: 9.4646

>AT53283 WP_000063160.1 NZ_CP011061:4838570-4838872 [Escherichia coli str. Sanji]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT53283 NZ_CP011061:4838570-4838872 [Escherichia coli str. Sanji]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9

Antitoxin

• AlphaFold DB: A0A080I7H7