Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4551511..4552008 Replicon chromosome
Accession NZ_CP011061
Organism Escherichia coli str. Sanji

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag Y979_RS22350 Protein ID WP_001173343.1
Coordinates 4551835..4552008 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag Y979_RS22345 Protein ID WP_000398619.1
Coordinates 4551511..4551807 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y979_RS22320 4548242..4548961 - 720 WP_000611288.1 two-component system response regulator DcuR -
Y979_RS22325 4548958..4550589 - 1632 WP_001216477.1 sensor histidine kinase -
Y979_RS22330 4550770..4551000 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
Y979_RS22335 4551012..4551284 + 273 WP_000405651.1 N-acetyltransferase -
Y979_RS22345 4551511..4551807 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
Y979_RS22350 4551835..4552008 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
Y979_RS22355 4552127..4553644 - 1518 WP_001295074.1 lysine--tRNA ligase -
Y979_RS22360 4553881..4555338 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T53280 WP_001173343.1 NZ_CP011061:4551835-4552008 [Escherichia coli str. Sanji]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T53280 NZ_CP011061:4551835-4552008 [Escherichia coli str. Sanji]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT53280 WP_000398619.1 NZ_CP011061:4551511-4551807 [Escherichia coli str. Sanji]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT53280 NZ_CP011061:4551511-4551807 [Escherichia coli str. Sanji]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References