Detailed information of TA system TA053276

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3233892..3234724
• Replicon: chromosome
• Accession: NZ_CP011061
• Organism: Escherichia coli str. Sanji

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: Y979_RS15870
• Protein ID: WP_000854765.1
• Coordinates: 3234350..3234724 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: Y979_RS15865
• Protein ID: WP_001295723.1
• Coordinates: 3233892..3234260 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y979_RS15825	3229556..3230236	+	681	WP_001282921.1	WYL domain-containing protein	-
Y979_RS15830	3230384..3231061	+	678	WP_001097302.1	hypothetical protein	-
Y979_RS15835	3231067..3231300	+	234	WP_001278293.1	DUF905 family protein	-
Y979_RS15840	3231390..3232208	+	819	WP_001175165.1	DUF945 domain-containing protein	-
Y979_RS15845	3232474..3232953	+	480	WP_057109541.1	antirestriction protein	-
Y979_RS15850	3232969..3233445	+	477	WP_001186774.1	RadC family protein	-
Y979_RS15855	3233508..3233729	+	222	WP_000692341.1	DUF987 domain-containing protein	-
Y979_RS15865	3233892..3234260	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
Y979_RS15870	3234350..3234724	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
Y979_RS15875	3234724..3234927	+	204	WP_001398797.1	hypothetical protein	-
Y979_RS15880	3234952..3235146	+	195	WP_024181941.1	DUF957 domain-containing protein	-
Y979_RS15885	3235231..3236073	+	843	WP_022645115.1	DUF4942 domain-containing protein	-
Y979_RS15890	3236354..3236890	-	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
Y979_RS15895	3236892..3238070	-	1179	WP_000094991.1	type II secretion system protein GspL	-
Y979_RS15900	3238067..3239044	-	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-
Y979_RS15905	3239047..3239646	-	600	WP_001255034.1	type II secretion system minor pseudopilin GspJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T53276 WP_000854765.1 NZ_CP011061:3234350-3234724 [Escherichia coli str. Sanji]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T53276 NZ_CP011061:3234350-3234724 [Escherichia coli str. Sanji]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT53276 WP_001295723.1 NZ_CP011061:3233892-3234260 [Escherichia coli str. Sanji]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT53276 NZ_CP011061:3233892-3234260 [Escherichia coli str. Sanji]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52