Detailed information of TA system TA053272

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2158354..2159189
• Replicon: chromosome
• Accession: NZ_CP011061
• Organism: Escherichia coli str. Sanji

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X9TM58
• Locus tag: Y979_RS10765
• Protein ID: WP_000854761.1
• Coordinates: 2158812..2159189 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: Y979_RS10760
• Protein ID: WP_001295723.1
• Coordinates: 2158354..2158722 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y979_RS10730	2155469..2155627	+	159	WP_001323397.1	DUF905 family protein	-
Y979_RS10735	2155782..2156600	+	819	WP_001234729.1	DUF945 domain-containing protein	-
Y979_RS10740	2156942..2157415	+	474	WP_000855059.1	antirestriction protein	-
Y979_RS10745	2157431..2157907	+	477	WP_001186774.1	RadC family protein	-
Y979_RS10750	2157970..2158191	+	222	WP_000692341.1	DUF987 domain-containing protein	-
Y979_RS10760	2158354..2158722	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
Y979_RS10765	2158812..2159189	+	378	WP_000854761.1	TA system toxin CbtA family protein	Toxin
Y979_RS10770	2159186..2159388	+	203	Protein_2051	hypothetical protein	-
Y979_RS30590	2159401..2159514	+	114	WP_001161660.1	DUF957 domain-containing protein	-
Y979_RS10780	2159949..2161484	-	1536	WP_001333339.1	IS66-like element ISEc22 family transposase	-
Y979_RS10785	2161533..2161880	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
Y979_RS10790	2161877..2162281	-	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
Y979_RS28035	2162775..2163164	+	390	WP_001445118.1	IS110 family transposase	-
Y979_RS30600	2163618..2163800	+	183	WP_001016348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14205.25 Da
• Isoelectric Point: 7.3249

>T53272 WP_000854761.1 NZ_CP011061:2158812-2159189 [Escherichia coli str. Sanji]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T53272 NZ_CP011061:2158812-2159189 [Escherichia coli str. Sanji]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT53272 WP_001295723.1 NZ_CP011061:2158354-2158722 [Escherichia coli str. Sanji]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT53272 NZ_CP011061:2158354-2158722 [Escherichia coli str. Sanji]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TM58

Antitoxin

• AlphaFold DB: S1NM52