Detailed information of TA system TA053260

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 382320..383014
• Replicon: chromosome
• Accession: NZ_CP011061
• Organism: Escherichia coli str. Sanji

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: Y979_RS01900
• Protein ID: WP_001094398.1
• Coordinates: 382646..383014 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: Y979_RS01895
• Protein ID: WP_077249034.1
• Coordinates: 382320..382625 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y979_RS01855	378270..378725	+	456	WP_000581506.1	hypothetical protein	-
Y979_RS30425	378826..379034	+	209	Protein_358	DUF905 family protein	-
Y979_RS01865	379135..379956	+	822	WP_001234565.1	DUF945 domain-containing protein	-
Y979_RS01875	380298..380771	+	474	WP_000855059.1	antirestriction protein	-
Y979_RS01880	380787..381263	+	477	WP_001424026.1	RadC family protein	-
Y979_RS01885	381332..381553	+	222	WP_000692345.1	DUF987 domain-containing protein	-
Y979_RS01890	381572..382216	+	645	WP_000086755.1	hypothetical protein	-
Y979_RS01895	382320..382625	+	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
Y979_RS01900	382646..383014	+	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
Y979_RS01905	383335..383673	-	339	Protein_366	LysR family transcriptional regulator	-
Y979_RS01910	383724..384680	-	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
Y979_RS01915	384690..386888	-	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
Y979_RS01920	386885..387841	-	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	366661..389559	22898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T53260 WP_001094398.1 NZ_CP011061:382646-383014 [Escherichia coli str. Sanji]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T53260 NZ_CP011061:382646-383014 [Escherichia coli str. Sanji]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT53260 WP_077249034.1 NZ_CP011061:382320-382625 [Escherichia coli str. Sanji]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT53260 NZ_CP011061:382320-382625 [Escherichia coli str. Sanji]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4